| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ECHDC1 |
| Uniprot No | Q9NTX5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-307aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MALKQEMAKS LLKTASLSGR TKLLHQTGLS LYSTSHGFYE EEVKKTLQQF PGGSIDLQKE DNGIGILTLN NPSRMNAFSG VMMLQLLEKV IELENWTEGK GLIVRGAKNT FSSGSDLNAV KSLGTPEDGM AVCMFMQNTL TRFMRLPLIS VALVQGWALG GGAEFTTACD FRLMTPESKI RFVHKEMGII PSWGGTTRLV EIIGSRQALK VLSGALKLDS KNALNIGMVE EVLQSSDETK SLEEAQEWLK QFIQGPPEVI RALKKSVCSG RELYLEEALQ NERDLLGTVW GGPANLEAIA KKGKFNKGGAEFTTACD FRLMTPESKI RFVHKEMGII PSWGGTTRLV EIIGSRQALK VLSGALKLDS KNALNIGMVE EVLQSSDETK SLEEAQEWLK QFIQGPPEVI RALKKSVCSG RELYLEEALQ NERDLLGTVW GGPANLEAIA KKGKFNK |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ECHDC1重组蛋白的3篇代表性文献的简要信息(注：文献为示例性概括，非真实存在)：
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1. **文献名称**：*"Cloning, Expression, and Functional Characterization of Recombinant ECHDC1 in Fatty Acid Metabolism"*
**作者**：Smith, J. et al.
**摘要**：该研究成功在大肠杆菌系统中表达并纯化了重组ECHDC1蛋白，证实其具有烯酰-CoA水解酶活性。实验表明，ECHDC1通过催化特定脂肪酸中间体的水合反应，参与调控细胞内的β-氧化通路，为代谢疾病研究提供了分子基础。
2. **文献名称**：*"Structural Insights into ECHDC1: Crystallographic Analysis of a Recombinant Human Enzyme"*
**作者**：Lee, S. et al.
**摘要**：本研究解析了重组人源ECHDC1蛋白的晶体结构(分辨率2.1 Å)，揭示了其活性中心的关键氨基酸残基及底物结合模式。结构分析表明，ECHDC1通过独特的催化机制区分不同链长的脂肪酸底物，为设计靶向抑制剂提供了依据。
3. **文献名称**：*"ECHDC1 Deficiency Promotes Lipid Accumulation: Evidence from Recombinant Protein-Based Cellular Assays"*
**作者**：Garcia, R. et al.
**摘要**：通过体外重组ECHDC1蛋白的功能实验，发现ECHDC1缺失会导致细胞内长链脂肪酸代谢障碍，并显著增加脂滴沉积。该研究提示ECHDC1可能作为肥胖或非酒精性脂肪肝的潜在治疗靶点。
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如需真实文献，建议通过PubMed或Web of Science检索关键词“ECHDC1 recombinant protein”或“ECHDC1 enoyl-CoA hydratase”获取最新研究。
**Background of ECHDC1 Recombinant Protein**
ECHDC1 (Enoyl-CoA Hydratase Domain Containing 1) is a protein encoded by the *ECHDC1* gene, which belongs to the hydrolase family. It is structurally characterized by an enoyl-CoA hydratase domain, suggesting a potential role in lipid metabolism, particularly in the β-oxidation pathway of fatty acids. ECHDC1 is evolutionarily conserved across species, highlighting its biological significance, though its precise enzymatic activity and substrates remain under investigation.
Recombinant ECHDC1 protein is engineered using expression systems such as *E. coli* or mammalian cells, enabling large-scale production for functional studies. It is often purified via affinity tags (e.g., His-tag) to ensure high purity and stability. Research indicates that ECHDC1 may interact with metabolic regulators, including peroxisomal proteins, linking it to cellular energy homeostasis and lipid processing.
Emerging studies associate ECHDC1 dysregulation with metabolic disorders, including obesity and type 2 diabetes, as well as certain cancers. For example, reduced ECHDC1 expression has been observed in hepatocellular carcinoma, suggesting a tumor-suppressive role. Additionally, its involvement in modulating lipid droplets and oxidative stress positions it as a potential therapeutic target.
The recombinant protein serves as a critical tool for in vitro assays, antibody development, and structural studies to elucidate its mechanism. Ongoing research aims to clarify its enzymatic function, regulatory networks, and pathophysiological relevance, offering insights into metabolic disease mechanisms and therapeutic strategies.
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