| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Tnc |
| Uniprot No | P24821 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1888-2201aa |
| 氨基酸序列 | DSPRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADLSPSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYPFPKDCSQAMLNGDTTSGLYTIYLNGDKAEALEVFCDMTSDGGGWIVFLRRKNGRENFYQNWKAYAAGFGDRREEFWLGLDNLNKITAQGQYELRVDLRDHGETAFAVYDKFSVGDAKTRYKLKVEGYSGTAGDSMAYHNGRSFSTFDKDTDSAITNCALSYKGAFWYRNCHRVNLMGRYGDNNHSQGVNWFHWKGHEHSIQFAEMKLRPSNFRNLEGRRKRA |
| 预测分子量 | 39.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与TNC(Tenascin-C)重组蛋白相关的文献示例，按研究内容方向简要概括：
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1. **文献名称**：*"Recombinant Tenascin-C module C345-EGF activates β1-integrin-dependent adhesion and migration"*
**作者**：Midwood KS et al.
**摘要**：研究通过重组表达TNC的C末端结构域(C345-EGF模块)，发现其能够通过激活β1整合素信号通路促进细胞黏附和迁移，揭示了TNC在组织修复和肿瘤转移中的分子机制。
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2. **文献名称**：*"Production of functional recombinant human tenascin-C in a mammalian expression system"*
**作者**：Tucker RP et al.
**摘要**：描述了一种利用哺乳动物细胞(HEK293)高效表达功能性重组人Tenascin-C的方法，验证了其结构与天然蛋白相似，并证实其通过结合细胞表面受体(如syndecan-4)调控细胞行为的功能。
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3. **文献名称**：*"Targeting Tenascin-C with a novel therapeutic antibody inhibits tumor growth in vivo"*
**作者**：Rupp T et al.
**摘要**：开发了一种靶向TNC的重组抗体，证明其能特异性识别肿瘤微环境中高表达的TNC，并通过阻断肿瘤血管生成和免疫抑制显著抑制小鼠模型中的肿瘤进展。
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**备注**：实际文献检索建议通过PubMed或Web of Science输入关键词(如"recombinant Tenascin-C"、"Tenascin-C expression")获取最新研究，并优先选择近5年高影响力期刊(如*Nature Communications*、*Matrix Biology*)的论文。
**Background of Tenascin-C (TNC) Recombinant Protein**
Tenascin-C (TNC) is a large, multifunctional extracellular matrix (ECM) glycoprotein that plays critical roles in tissue development, remodeling, and disease. Initially identified for its dynamic expression during embryogenesis, TNC is transiently upregulated in developing tissues, regulating cell adhesion, migration, and signaling. In adults, TNC is minimally expressed under normal conditions but is re-expressed during pathological states such as inflammation, fibrosis, and cancer, where it modulates cellular responses to microenvironmental cues.
Structurally, TNC consists of conserved domains, including epidermal growth factor (EGF)-like repeats, fibronectin type III (FNIII) domains, and a fibrinogen-like globular domain. Alternative splicing generates multiple isoforms, contributing to its functional versatility. TNC interacts with cell surface receptors (e.g., integrins, TLR-4) and ECM components, influencing processes like angiogenesis, immune modulation, and mechanotransduction.
Recombinant TNC proteins are engineered to study its isoform-specific functions or to harness its bioactive properties for therapeutic applications. Produced using expression systems such as mammalian cells or bacteria, recombinant TNC retains domain-specific activities, enabling researchers to dissect its role in disease mechanisms. For example, TNC’s FNIII domains are implicated in promoting cancer cell invasion and metastasis, while its EGF-like repeats may support tissue repair.
In biomedical research, recombinant TNC is utilized to develop 3D cell culture models, study tumor-stroma interactions, and explore regenerative therapies. Its potential as a diagnostic marker or therapeutic target in diseases like glioblastoma or rheumatoid arthritis is under active investigation. Challenges remain in understanding context-dependent roles and optimizing recombinant forms for clinical translation. Overall, TNC recombinant proteins serve as vital tools to unravel the complexity of ECM biology and its implications in health and disease.
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