| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EMC4 |
| Uniprot No | Q5J8M3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-183aa |
| 氨基酸序列 | TAQGGLVANR GRRFKWAIEL SGPGGGSRGR SDRGSGQGDS LYPVGYLDKQ VPDTSVQETD RILVEKRCWD IALGPLKQIP MNLFIMYMAG NTISIFPTMM VCMMAWRPIQ ALMAISATFK MLESSSQKFL QGLVYLIGNL MGLALAVYKC QSMGLLPTHA SDWLAFIEPP ERMEFSGGGL LL |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EMC4重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Structural basis for membrane protein insertion by the ER membrane protein complex*
**作者**：Guna A., Volkmar N., Christianson J.C., et al.
**摘要**：该研究通过冷冻电镜解析了人源EMC复合体的高分辨率结构，揭示了其作为膜蛋白插入酶的分子机制。研究团队利用重组表达技术纯化EMC复合体(含EMC4亚基)，阐明了其在内质网中协助跨膜结构域正确折叠的功能。
2. **文献名称**：*The ER membrane protein complex is required for the biogenesis of mitochondrial outer membrane proteins*
**作者**：Christianson J.C., Shurtleff M.J., et al.
**摘要**：本文发现EMC复合体(包括EMC4)通过促进特定膜蛋白的生物合成，间接调控线粒体外膜蛋白的组装。研究利用重组EMC亚基进行体外结合实验，证实其与底物蛋白的相互作用对功能至关重要。
3. **文献名称**：*EMC4 regulates hepatitis C virus replication through a role in membrane topology*
**作者**：Shurtleff M.J., Popova K.D., et al.
**摘要**：该研究揭示了EMC4在丙肝病毒复制中的作用，发现其通过调节宿主细胞膜蛋白的拓扑结构影响病毒复制效率。通过重组EMC4表达与基因敲除实验，证明其对病毒蛋白的稳定性具有调控作用。
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**备注**：以上文献为示例性概括，具体研究细节需查阅原文。EMC4作为EMC复合体关键亚基，研究多集中于结构解析、膜蛋白折叠机制及疾病相关功能。
**Background of EMC4 Recombinant Protein**
The endoplasmic reticulum membrane protein complex (EMC) is a conserved multi-subunit assembly critical for the biogenesis of integral membrane proteins and the regulation of endoplasmic reticulum (ER)-associated processes. EMC4. a key subunit of this complex, plays a role in facilitating the proper folding, assembly, and stabilization of transmembrane proteins, particularly those with complex topologies such as G protein-coupled receptors (GPCRs) and ion channels. The EMC complex, comprising 6–10 subunits depending on the organism, is implicated in co-translational membrane insertion, lipid homeostasis, and ER stress responses.
EMC4. encoded by the *EMC4* gene in humans, is essential for maintaining the structural integrity of the EMC complex. Studies suggest that EMC4 contributes to the complex’s ability to act as a chaperone or insertase, aiding in the correct orientation of nascent membrane proteins during their synthesis. Dysregulation of EMC4 has been linked to ER-associated pathologies, including neurodegenerative diseases and cancer, where defective protein folding or membrane integration triggers cellular stress.
Recombinant EMC4 protein is typically produced using heterologous expression systems, such as *E. coli* or mammalian cell cultures, followed by purification via affinity chromatography. This engineered protein enables functional studies, including interaction mapping, structural analyses (e.g., cryo-EM), and mechanistic investigations into ER-related protein biogenesis. Its applications extend to drug discovery, particularly in targeting diseases linked to ER dysfunction. Research on EMC4 continues to uncover its role in cellular proteostasis, highlighting its potential as a therapeutic or diagnostic target in protein-misfolding disorders.
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