| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CNGA4 |
| Uniprot No | Q8IV77 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-575aa |
| 氨基酸序列 | MSQDTKVKTTESSPPAPSKARKLLPVLDPSGDYYYWWLNTMVFPVMYNLI ILVCRACFPDLQHGYLVAWLVLDYTSDLLYLLDMVVRFHTGFLEQGILVV DKGRISSRYVRTWSFFLDLASLMPTDVVYVRLGPHTPTLRLNRFLRAPRL FEAFDRTETRTAYPNAFRIAKLMLYIFVVIHWNSCLYFALSRYLGFGRDA WVYPDPAQPGFERLRRQYLYSFYFSTLILTTVGDTPPPAREEEYLFMVGD FLLAVMGFATIMGSMSSVIYNMNTADAAFYPDHALVKKYMKLQHVNRKLE RRVIDWYQHLQINKKMTNEVAILQHLPERLRAEVAVSVHLSTLSRVQIFQ NCEASLLEELVLKLQPQTYSPGEYVCRKGDIGQEMYIIREGQLAVVADDG ITQYAVLGAGLYFGEISIINIKGNMSGNRRTANIKSLGYSDLFCLSKEDL REVLSEYPQAQTIMEEKGREILLKMNKLDVNAEAAEIALQEATESRLRGL DQQLDDLQTKFARLLAELESSALKIAYRIERLEWQTREWPMPEDLAEADD EGEPEEGTSKDEEGRASQEGPPGPE |
| 预测分子量 | 66 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CNGA4重组蛋白的3篇参考文献示例(注：文献为模拟概括，实际引用需核实原文)：
1. **文献名称**：*Structural insights into the gating mechanism of CNGA4 channel subunits*
**作者**：Smith A, et al.
**摘要**：本研究通过重组表达CNGA4蛋白，结合冷冻电镜技术解析其三维结构，揭示了该亚基在环核苷酸门控通道中的构象变化及门控调节机制。
2. **文献名称**：*Functional interaction between CNGA4 and CNGB1b subunits in olfactory cyclic nucleotide-gated channels*
**作者**：Brown C, et al.
**摘要**：利用共表达CNGA4和CNGB1b重组蛋白，证明CNGA4可调控通道对cAMP/cGMP的敏感性，并影响离子选择性和电流动力学特性。
3. **文献名称**：*Role of CNGA4 in cardiac pacemaker activity: Insights from recombinant protein studies*
**作者**：Zhang L, et al.
**摘要**：通过体外表达CNGA4重组蛋白，发现其参与心脏HCN通道的调节，揭示CNGA4异常表达可能导致心律失常的分子机制。
如需具体文献，建议在PubMed或Web of Science中检索关键词“CNGA4 recombinant protein”获取最新研究。
**Background of CNGA4 Recombinant Protein**
Cyclic nucleotide-gated channel alpha-4 subunit (CNGA4) is a critical component of cyclic nucleotide-gated (CNG) channels, which play essential roles in sensory signal transduction, particularly in olfactory and visual systems. CNG channels are non-selective cation channels activated by cyclic nucleotides like cAMP or cGMP. In mammals, CNGA4 is predominantly expressed in olfactory sensory neurons, where it forms heterotetrameric complexes with other subunits (e.g., CNGA2 and CNGB1b) to mediate odorant-induced signaling. This channel activation leads to membrane depolarization, converting chemical signals into electrical responses.
CNGA4 is unique for its modulatory role. Unlike principal subunits (e.g., CNGA2), CNGA4 lacks intrinsic channel activity but enhances channel sensitivity to cAMP and accelerates channel gating kinetics. It also contributes to calcium-dependent feedback regulation, fine-tuning olfactory adaptation. Dysregulation of CNGA4 has been linked to impaired olfaction and neurodegenerative conditions.
Recombinant CNGA4 protein is produced using expression systems like *E. coli* or mammalian cell lines (e.g., HEK293) to study its structure-function relationships, interactions with partner subunits, and mechanisms underlying channel modulation. Purification often involves affinity tags (e.g., His-tag) followed by chromatography. Its applications span *in vitro* electrophysiology, structural biology (e.g., cryo-EM), and drug screening for therapies targeting CNG channelopathies.
Research on CNGA4 recombinant protein advances understanding of sensory signaling pathologies and aids in developing targeted therapeutics for olfactory dysfunction or retinal diseases linked to CNG channel anomalies.
×
