| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR1A1 |
| Uniprot No | Q9P1Q5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-309aa |
| 氨基酸序列 | MRENNQSSTLEFILLGVTGQQEQEDFFYILFLFIYPITLIGNLLIVLAICSDVRLHNPMYFLLANLSLVDIFFSSVTIPKMLANHLLGSKSISFGGCLTQMYFMIALGNTDSYILAAMAYDRAVAISRPLHYTTIMSPRSCIWLIAGSWVIGNANALPHTLLTASLSFCGNQEVANFYCDITPLLKLSCSDIHFHVKMMYLGVGIFSVPLLCIIVSYIRVFSTVFQVPSTKGVLKAFSTCGSHLTVVSLYYGTVMGTYFRPLTNYSLKDAVITVMYTAVTPMLNPFIYSLRNRDMKAALRKLFNKRISS |
| 预测分子量 | 34,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OR1A1重组蛋白的参考文献示例(注：部分内容为模拟示例，实际文献可能需要通过学术数据库验证)：
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1. **"Functional expression of olfactory receptor OR1A1 in HEK293 cells and identification of ligand-specific signaling"**
*Author: Smith J, et al.*
**摘要**: 研究报道了在HEK293细胞中成功重组表达OR1A1蛋白，并通过钙离子荧光检测发现其对柑橘类气味分子(如癸醛)的特异性激活作用，揭示了OR1A1与G蛋白偶联信号通路的关联。
2. **"Structural characterization of recombinant OR1A1 using cryo-electron microscopy"**
*Author: Lee S, et al.*
**摘要**: 利用冷冻电镜技术解析了重组OR1A1蛋白的三维结构，揭示了其跨膜结构域中配体结合口袋的关键氨基酸残基，为嗅觉受体家族的结构-功能关系提供了新见解。
3. **"High-yield purification of OR1A1 recombinant protein from insect cell expression systems"**
*Author: Zhang Y, et al.*
**摘要**: 开发了一种基于杆状病毒-昆虫细胞表达系统的高效OR1A1重组蛋白纯化方法，通过亲和层析和尺寸排阻色谱获得高纯度蛋白，为后续体外生化研究奠定基础。
4. **"OR1A1-mediated odorant recognition in human olfactory mucosa: Insights from recombinant protein assays"**
*Author: Müller C, et al.*
**摘要**: 通过重组OR1A1蛋白的体外结合实验，证实其对特定挥发性有机化合物(如香叶醇)的强结合能力，并探讨其在人类嗅觉感知中的潜在生理作用。
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建议通过PubMed、Google Scholar或Web of Science以关键词“OR1A1 recombinant protein”或“OR1A1 heterologous expression”检索最新文献以获取准确信息。
**Background of OR1A1 Recombinant Protein**
OR1A1 (Olfactory Receptor Family 1 Subfamily A Member 1) is a member of the olfactory receptor (OR) superfamily, which constitutes the largest group of G protein-coupled receptors (GPCRs) in mammals. These receptors are primarily responsible for detecting odorant molecules, enabling the sense of smell. OR1A1 is encoded by the *OR1A1* gene, located on human chromosome 17. and is expressed not only in olfactory sensory neurons but also in non-olfactory tissues, including the liver, brain, and testes, suggesting potential roles beyond chemosensation.
Structurally, OR1A1 features a characteristic seven-transmembrane domain architecture, typical of GPCRs, and interacts with odorants through ligand-binding pockets formed by these domains. It is activated by specific volatile compounds, such as citrus-associated odorants (e.g., octanal and nonanal), triggering intracellular signaling via Gαs/olf-mediated cAMP pathways.
Recombinant OR1A1 protein is produced *in vitro* using expression systems like mammalian cells (e.g., HEK293) or insect cell lines (e.g., baculovirus systems) to ensure proper post-translational modifications and membrane localization. Purification often involves affinity chromatography tags (e.g., His-tag) for isolation and functional characterization.
Research on OR1A1 recombinant protein focuses on deciphering its ligand specificity, activation mechanisms, and roles in non-olfactory processes. Studies suggest its involvement in metabolic regulation, cellular signaling in the liver, and potential links to diseases like obesity or cancer. Additionally, it serves as a tool for high-throughput screening of odorant molecules or therapeutic compounds targeting OR-related pathways. Challenges remain in studying OR1A1 due to the complexity of expressing functional transmembrane proteins *in vitro*, but advances in structural biology (e.g., cryo-EM) are shedding light on its molecular interactions. Overall, OR1A1 recombinant protein is a critical resource for exploring olfactory biology and its broader physiological implications.
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