| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EIF3E |
| Uniprot No | P60228 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-445aa |
| 氨基酸序列 | MAEYDLTTRIAHFLDRHLVFPLLEFLSVKEIYNEKELLQGKLDLLSDTNMVDFAMDVYKNLYSDDIPHALREKRTTVVAQLKQLQAETEPIVKMFEDPETTRQMQSTRDGRMLFDYLADKHGFRQEYLDTLYRYAKFQYECGNYSGAAEYLYFFRVLVPATDRNALSSLWGKLASEILMQNWDAAMEDLTRLKETIDNNSVSSPLQSLQQRTWLIHWSLFVFFNHPKGRDNIIDLFLYQPQYLNAIQTMCPHILRYLTTAVITNKDVRKRRQVLKDLVKVIQQESYTYKDPITEFVECLYVNFDFDGAQKKLRECESVLVNDFFLVACLEDFIENARLFIFETFCRIHQCISINMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNNAVSPYQQVIEKTKSLSFRSQMLAMNIEKKLNQNSRSEAPNWATQDSGFY |
| 预测分子量 | 59.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EIF3E重组蛋白的3篇参考文献及其摘要概览：
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1. **文献名称**：*"EIF3E interacts with the PP2A phosphatase complex to regulate translation in cancer cells"*
**作者**：Dong, Z., et al.
**摘要**：本研究揭示了EIF3E通过结合PP2A磷酸酶复合体调控mRNA翻译的分子机制，发现EIF3E重组蛋白在肺癌细胞中抑制肿瘤生长，并证明其低表达与患者预后不良相关。
2. **文献名称**：*"Expression and purification of functional recombinant human EIF3E for structural studies"*
**作者**：Smith, J.R., et al.
**摘要**：开发了基于大肠杆菌的重组人EIF3E高效表达与纯化系统，通过X射线晶体学解析其三维结构，为研究EIF3E在翻译起始复合体中的功能提供基础。
3. **文献名称**：*"EIF3E depletion disrupts the assembly of the eukaryotic translation initiation factor 3 complex"*
**作者**：Zhang, Y., et al.
**摘要**：利用CRISPR技术敲低EIF3E后，发现其重组蛋白无法有效整合到翻译起始复合体中，导致全局蛋白质合成速率下降，证实EIF3E对复合体稳定性的关键作用。
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(注：以上文献为示例性内容，实际引用时需根据具体论文核实信息。)
**Background of EIF3E Recombinant Protein**
Eukaryotic Initiation Factor 3 Subunit E (EIF3E) is a critical component of the EIF3 complex, a multi-protein assembly essential for initiating cap-dependent translation in eukaryotic cells. The EIF3 complex coordinates ribosome recruitment to mRNA, scans for the start codon, and regulates the overall efficiency of protein synthesis. EIF3E, specifically, plays a structural and functional role in stabilizing the EIF3 holocomplex and mediating interactions with other initiation factors or regulatory molecules. Dysregulation of EIF3E has been implicated in various pathologies, including cancers, where its overexpression or deletion may alter translational programs to favor tumor growth or metastasis.
Recombinant EIF3E protein is produced using biotechnological methods, typically through heterologous expression in bacterial, insect, or mammalian systems. This involves cloning the EIF3E gene into an expression vector, followed by purification via affinity chromatography (e.g., His-tag systems) to ensure high purity and functionality. The recombinant protein retains the native structure and activity of endogenous EIF3E, enabling researchers to study its biochemical properties, molecular interactions, and role in translation regulation under controlled conditions.
Studies utilizing EIF3E recombinant protein have advanced our understanding of its involvement in cellular stress responses, viral infection mechanisms (where pathogens hijack host translation machinery), and oncogenic signaling pathways. Additionally, it serves as a valuable tool for screening therapeutic compounds targeting translation initiation in diseases like cancer or neurodegenerative disorders. By dissecting EIF3E's role at a molecular level, researchers aim to uncover novel strategies for modulating protein synthesis in pathological contexts.
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