| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM110B |
| Uniprot No | Q8TC76 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-370aa |
| 氨基酸序列 | MPTETLQTGSMVKPVSPAGTFTSAVPLRILNKGPDYFRRQAEPNPKRLSAVERLEADKAKYVKSQEVINAKQEPVKPAVLAKPPVCPAAKRALGSPTLKVFGNHAKTESGVQRENLKLEILKNIINSSEGSSSGSGHKHSSRNWPPHRSEATDLHRHSFAESLKVYPTQGRRSPQEGGSHVGRRLLEQSAESFLHVSHSSSDIRKVTSVKPLKAIPCSSSAPPLPPKPKIAAIASMKSPEADPVEPACGVSRRPSLQRSKSDLSDRYFRVDADVERFFNYCGLDPEELENLGMENFARANSDIISLNFRSASMISSDCEQSQDSNSDLRNDDSANDRVPYGISAIERNARIIKWLYSIKQARESQKVSHV |
| 预测分子量 | 44.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FAM110B重组蛋白的3篇虚构参考文献示例，基于类似领域研究的常见方向编写：
---
1. **文献名称**: *"FAM110B interacts with microtubule-associated proteins and regulates mitotic progression"*
**作者**: Zhang L, et al.
**摘要**: 本研究通过免疫共沉淀和质谱分析发现FAM110B重组蛋白与微管结合蛋白(如TPX2)相互作用，调控有丝分裂中纺锤体组装。敲低FAM110B导致染色体排列异常，提示其在细胞周期中的关键作用。
2. **文献名称**: *"Expression and functional characterization of recombinant FAM110B in cancer cell lines"*
**作者**: Patel R, et al.
**摘要**: 作者利用大肠杆菌系统表达并纯化FAM110B重组蛋白，发现其过表达抑制乳腺癌细胞迁移。蛋白质组学分析表明FAM110B通过调控Wnt/β-catenin通路影响肿瘤进展。
3. **文献名称**: *"Structural insights into FAM110B’s role in centrosome amplification"*
**作者**: Kim S, et al.
**摘要**: 通过冷冻电镜解析FAM110B重组蛋白结构，揭示其C端结构域与中心体蛋白CEP192结合，调控中心体复制。突变实验证实该互作异常可导致基因组不稳定性。
---
**说明**：以上内容为模拟生成，实际文献需通过PubMed或Web of Science等平台检索。若需真实文献，建议结合关键词“FAM110B recombinant protein”或“FAM110B function”查询近年发表论文。
**Background on FAM110B Recombinant Protein**
FAM110B (Family with sequence similarity 110 member B) is a protein-coding gene located on human chromosome 9. with limited functional characterization in current literature. It belongs to the FAM110 family, which includes proteins with conserved domains potentially involved in cell cycle regulation, though their exact mechanisms remain unclear. FAM110B is hypothesized to play roles in cellular processes such as mitosis, cytoskeletal organization, or signal transduction, supported by its interaction with microtubule-associated proteins and its nuclear-cytoplasmic localization. Dysregulation of FAM110B has been loosely associated with cancers, including gliomas and prostate cancer, though its oncogenic or tumor-suppressive role requires further validation.
Recombinant FAM110B protein is produced using heterologous expression systems (e.g., *E. coli*, mammalian cells) to enable functional studies. The protein is typically engineered with tags (e.g., His-tag, GST) for purification and detection. Its recombinant form aids in investigating interactions with binding partners, structural features (e.g., coiled-coil domains), and post-translational modifications. Researchers utilize it to generate antibodies, map epitopes, or study its role in cell proliferation assays and disease models.
Current challenges include clarifying FAM110B's physiological substrates, signaling pathways, and tissue-specific functions. Its recombinant version is pivotal for addressing these gaps, offering tools for biochemical assays (e.g., pull-down, kinase assays) and structural studies (e.g., crystallography). Further exploration of FAM110B could unveil its potential as a biomarker or therapeutic target, particularly in cancers where its expression correlates with prognosis. Overall, recombinant FAM110B serves as a critical reagent to decode its biological significance and therapeutic relevance.
×
