| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPC6 |
| Uniprot No | Q9Y625 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-529aa |
| 氨基酸序列 | DVKARSCGEVRQAYGAKGFSLADIPYQEIAGEHLRICPQEYTCCTTEMEDKLSQQSKLEFENLVEETSHFVRTTFVSRHKKFDEFFRELLENAEKSLNDMFVRTYGMLYMQNSEVFQDLFTELKRYYTGGNVNLEEMLNDFWARLLERMFQLINPQYHFSEDYLECVSKYTDQLKPFGDVPRKLKIQVTRAFIAARTFVQGLTVGREVANRVSKVSPTPGCIRALMKMLYCPYCRGLPTVRPCNNYCLNVMKGCLANQADLDTEWNLFIDAMLLVAERLEGPFNIESVMDPIDVKISEAIMNMQENSMQVSAKVFQGCGQPKPAPALRSARSAPENFNTRFRPYNPEERPTTAAGTSLDRLVTDIKEKLKLSKKVWSALPYTICKDESVTAGTSNEEECWNGHSKARYLPEIMNDGLTNQINNPEVDVDITRPDTFIRQQIMALRVMTNKLKNAYNGNDVNFQDTSDESSGSGSGSGCMDDVCPTEFEFVTTEAPAVDPDRREVDS |
| 预测分子量 | 70.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPC6重组蛋白的3篇示例文献(注：内容为模拟概括，基于领域常见研究方向)：
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1. **文献名称**：*Recombinant Expression and Functional Characterization of Human Glypican-6 in Osteoblast Differentiation*
**作者**：Chen, L. et al.
**摘要**：本研究通过哺乳动物表达系统成功制备了重组人GPC6蛋白，并验证其促进成骨细胞分化的功能。实验表明，重组GPC6通过调控BMP-2信号通路增强骨形成，为骨骼疾病治疗提供潜在靶点。
2. **文献名称**：*Structural Analysis of Glypican-6 Reveals Its Role in Wnt/β-catenin Signaling Activation*
**作者**：Wang, Y. et al.
**摘要**：利用昆虫细胞系统表达并纯化GPC6重组蛋白，结合X射线晶体学解析其三维结构。研究发现，GPC6通过结合Wnt配体增强β-catenin信号，可能参与癌症进展。
3. **文献名称**：*High-Yield Production of Recombinant GPC6 in E. coli and Its Application in Antibody Development*
**作者**：Kim, S. et al.
**摘要**：开发了一种基于大肠杆菌的重组GPC6高效表达与纯化策略，并利用该蛋白成功制备了特异性抗体，为GPC6相关诊断试剂的开发奠定基础。
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如需真实文献，建议通过PubMed或Web of Science检索关键词“recombinant GPC6”或“Glypican-6 expression”获取具体信息。
**Background of GPC6 Recombinant Protein**
Glypican-6 (GPC6) is a member of the glypican family of heparan sulfate proteoglycans (HSPGs), which are anchored to the cell membrane via glycosylphosphatidylinositol (GPI) linkages. These proteins play critical roles in modulating signaling pathways by interacting with growth factors, receptors, and extracellular matrix components. GPC6 is predominantly expressed during embryonic development, particularly in skeletal and neural tissues, and is implicated in regulating cellular proliferation, differentiation, and tissue morphogenesis.
Structurally, GPC6 consists of a core protein with conserved cysteine residues and heparan sulfate (HS) chains attached near the C-terminal. The HS chains enable GPC6 to bind ligands such as Wnt, Hedgehog, and bone morphogenetic proteins (BMPs), influencing signaling cascades like Wnt/β-catenin and Hedgehog pathways. Dysregulation of GPC6 has been linked to developmental disorders, including skeletal dysplasia, and pathological conditions such as cancer. For instance, GPC6 acts as a tumor suppressor in certain cancers but may promote metastasis in others, highlighting its context-dependent roles.
Recombinant GPC6 protein is engineered using expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications, particularly HS chain attachment. This purified protein serves as a tool to study GPC6-ligand interactions, signaling mechanisms, and therapeutic potential. Recent studies explore its utility in bone regeneration, cancer therapy, and diagnostic biomarker development. By elucidating GPC6's structure-function relationships and disease associations, researchers aim to harness its biological significance for targeted interventions in developmental and oncological disorders.
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