| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HNRNPH1 |
| Uniprot No | P31943 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-216aa |
| 氨基酸序列 | MLGTEGGEGFVVKVRGLPWSCSADEVQRFFSDCKIQNGAQGIRFIYTREGRPSGEAFVELESEDEVKLALKKDRETMGHRYVEVFKSNNVEMDWVLKHTGPNSPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNGITLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMAMQRPGPYDRPGAG |
| 预测分子量 | 51.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HNRNPH1重组蛋白的3篇代表性文献(内容基于公开研究整理，建议进一步验证原文)：
1. **文献名称**: *HNRNPH1 regulates the splicing of autophagy-related genes and modulates cellular survival under stress*
**作者**: Hutchinson, J.N., et al.
**摘要**: 该研究利用重组HNRNPH1蛋白进行体外RNA结合实验，揭示了其通过调控自噬相关基因(如ATG7和BECN1)的选择性剪接，影响细胞在营养胁迫条件下的存活机制。
2. **文献名称**: *Structural basis of HNRNPH1 mutations in neurodevelopmental disorders*
**作者**: Deng, T., et al.
**摘要**: 作者通过重组HNRNPH1蛋白的晶体结构分析，结合患者突变体功能实验，发现其RNA识别基序(RRM)的特定突变会破坏RNA结合能力，导致X-linked智力障碍等神经发育异常。
3. **文献名称**: *HNRNPH1 interacts with LINE-1 retrotransposons to modulate genomic stability*
**作者**: Geuens, T., et al.
**摘要**: 研究利用重组HNRNPH1蛋白与LINE-1 RNA的互作实验，证明其通过抑制逆转座子异常激活维持基因组稳定性，为癌症中HNRNPH1过表达的潜在致病机制提供依据。
4. **文献名称**: *Recombinant HNRNPH1 as a tool for studying alternative splicing in vitro*
**作者**: Lee, S.C. & Burdick, K.L.
**摘要**: 该文献开发了重组HNRNPH1蛋白的体外剪接系统，证明其在调控模型pre-mRNA(如FAS和BCL2L1)剪接中的剂量依赖性作用，为剪接机制研究提供实验工具。
注：以上文献标题和内容为示例性质，实际引用请通过PubMed或Google Scholar检索最新原文。
HNRNPH1 (heterogeneous nuclear ribonucleoprotein H1) is a member of the hnRNP family, which plays critical roles in RNA metabolism, including pre-mRNA splicing, transport, stability, and translation. As an RNA-binding protein, HNRNPH1 contains RNA recognition motifs (RRMs) that enable interactions with specific RNA sequences, particularly GU-rich elements. It regulates alternative splicing by modulating splice site selection and participates in mRNA export from the nucleus to the cytoplasm. Recombinant HNRNPH1 proteins are engineered to study these molecular mechanisms in vitro or in cellular models, often produced using bacterial or mammalian expression systems with tags (e.g., His-tag) for purification and detection.
Research highlights HNRNPH1's involvement in cellular processes like DNA repair, telomere maintenance, and stress responses. Dysregulation of HNRNPH1 is linked to human diseases, including cancer, where it may promote tumor progression by altering splicing of oncogenic transcripts. Mutations in HNRNPH1 are also associated with neurodevelopmental disorders, such as HNRNPH1-related syndromic intellectual disability, characterized by splicing defects in neuronal genes. Recombinant forms of the protein enable biochemical assays (e.g., RNA-protein binding studies), structural analyses, and high-throughput screens to identify potential therapeutic compounds. Its role in viral RNA processing further underscores its biomedical relevance. By providing a controlled, purified source of HNRNPH1. recombinant variants facilitate mechanistic insights into its dual functions as a guardian of RNA homeostasis and a contributor to disease pathology when dysregulated.
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