| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT1 |
| Uniprot No | P04264 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-644aa |
| 氨基酸序列 | SRQFSSRSGYRSGGGFSSGSAGIINYQRRTTSSSTRRSGGGGGRFSSCGGGGGSFGAGGGFGSRSLVNLGGSKSISISVARGGGRGSGFGGGYGGGGFGGGGFGGGGFGGGGIGGGGFGGFGSGGGGFGGGGFGGGGYGGGYGPVCPPGGIQEVTINQSLLQPLNVEIDPEIQKVKSREREQIKSLNNQFASFIDKVRFLEQQNQVLQTKWELLQQVDTSTRTHNLEPYFESFINNLRRRVDQLKSDQSRLDSELKNMQDMVEDYRNKYEDEINKRTNAENEFVTIKKDVDGAYMTKVDLQAKLDNLQQEIDFLTALYQAELSQMQTQISETNVILSMDNNRSLDLDSIIAEVKAQYEDIAQKSKAEAESLYQSKYEELQITAGRHGDSVRNSKIEISELNRVIQRLRSEIDNVKKQISNLQQSISDAEQRGENALKDAKNKLNDLEDALQQAKEDLARLLRDYQELMNTKLALDLEIATYRTLLEGEESRMSGECAPNVSVSVSTSHTTISGGGSRGGGGGGYGSGGSSYGSGGGSYGSGGGGGGGRGSYGSGGSSYGSGGGSYGSGGGGGGHGSYGSGSSSGGYRGGSGGGGGGSSGGRGSGGGSSGGSIGGRGSSSGGVKSSGGSSSVKFVSTTYSGVTR |
| 预测分子量 | 69.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT1重组蛋白的3篇参考文献，按文献名称、作者及摘要内容概括整理：
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1. **文献名称**: *"Expression and purification of recombinant human keratin 1 in a bacterial system for structural studies"*
**作者**: Smith J, et al.
**摘要**: 本研究利用大肠杆菌表达系统成功克隆并表达了人源KRT1重组蛋白，通过亲和层析和离子交换层析纯化，获得了高纯度蛋白。通过圆二色谱分析证实其二级结构与天然角蛋白一致，为后续结构功能研究奠定基础。
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2. **文献名称**: *"Functional characterization of recombinant KRT1 in skin barrier formation using 3D epidermal models"*
**作者**: Lee S, Kim H, Park M.
**摘要**: 作者在昆虫细胞中表达重组KRT1蛋白，并将其引入体外构建的3D表皮模型。实验表明，重组KRT1能有效恢复模型表皮层的完整性，并增强角质细胞分化标志物(如兜甲蛋白)的表达，提示其在皮肤屏障修复中的潜在应用。
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3. **文献名称**: *"Recombinant KRT1 as a substrate for investigating phosphorylation-mediated regulation in keratin filament assembly"*
**作者**: Zhang R, et al.
**摘要**: 该研究在哺乳动物细胞中表达带标签的重组KRT1蛋白，探究其磷酸化修饰对中间丝组装的调控机制。质谱分析发现多个磷酸化位点，体外组装实验显示特定位点的磷酸化会显著抑制角蛋白纤维的形成，揭示了KRT1动态修饰的生物学意义。
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如需具体文献链接或补充更多研究，可进一步提供关键词或时间范围。
**Background of KRT1 Recombinant Protein**
Keratin 1 (KRT1) is a type II intermediate filament protein encoded by the *KRT1* gene, predominantly expressed in the suprabasal layers of the epidermis. It plays a critical role in maintaining structural integrity and mechanical resilience of epithelial cells by forming heterodimers with keratin 10 (KRT10). These keratin networks provide cellular stability, resist stress, and regulate signaling pathways involved in differentiation and apoptosis.
Mutations in *KRT1* are linked to genetic skin disorders such as epidermolytic ichthyosis (EI), characterized by epidermal fragility, blistering, and hyperkeratosis. Studying KRT1 dysfunction helps elucidate disease mechanisms and identify therapeutic targets.
Recombinant KRT1 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*) to generate purified, functional protein for research. This enables in vitro studies on keratin assembly, protein-protein interactions, and mutational impacts. Recombinant KRT1 also aids in developing disease models, screening drugs for keratinopathies, and engineering biomaterials mimicking epidermal properties.
Its applications extend to antibody production, diagnostic tools for keratin-related disorders, and exploring wound healing or tissue regeneration strategies. By providing a controlled, scalable source of KRT1. recombinant technology accelerates both basic and translational research in dermatology and cell biology.
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