| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MT1X |
| Uniprot No | P80297 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-59aa |
| 氨基酸序列 | MDPNCSCSPVGSCACAGSCKCKECKCTSCKKSCCSCCPVGCAKCAQGCICKGTSDKCSC |
| 预测分子量 | 32.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与MT1X重组蛋白相关的文献摘要(模拟数据，仅供参考)：
1. **文献名称**: "Expression and Purification of Recombinant Human MT1X in E. coli for Metal Binding Studies"
**作者**: Li Y, et al.
**摘要**: 本研究成功构建了MT1X基因原核表达载体，利用大肠杆菌系统高效表达可溶性重组MT1X蛋白，并验证其结合锌、镉离子的能力，为金属解毒机制研究提供工具。
2. **文献名称**: "MT1X Overexpression Attenuates Oxidative Stress in Hepatocytes: Insights from Recombinant Protein Delivery"
**作者**: Gonzalez S, et al.
**摘要**: 通过哺乳动物细胞表达纯化MT1X重组蛋白，证明外源性MT1X可增强肝细胞对H₂O₂诱导氧化损伤的抵抗，提示其潜在抗氧化治疗价值。
3. **文献名称**: "Structural Characterization of Recombinant MT1X by NMR Spectroscopy"
**作者**: Müller P, et al.
**摘要**: 首次报道MT1X重组蛋白的核磁共振结构解析，揭示其α/β结构域动态构象变化与金属离子配位特异性，为设计金属调控分子奠定基础。
注：以上文献为模拟示例，实际引用需查询具体数据库(如PubMed/Web of Science)并核实原文。建议使用关键词 "MT1X recombinant protein expression" 或 "metallothionein 1X purification" 进行检索。
**Background of MT1X Recombinant Protein**
MT1X (Metallothionein 1X) is a member of the metallothionein (MT) family, a group of small, cysteine-rich proteins that bind metal ions and play critical roles in metal homeostasis, detoxification, and cellular protection against oxidative stress. The MT1X gene, located on human chromosome 16q13. encodes a protein that shares structural and functional similarities with other MT isoforms, characterized by a high cysteine content (~30%) and a lack of aromatic amino acids.
MT1X is primarily expressed in tissues such as the liver, kidney, and intestines, where it regulates the absorption, storage, and transport of essential metals like zinc and copper, while also detoxifying heavy metals such as cadmium and mercury. Its expression is tightly regulated by metal ions, glucocorticoids, and oxidative stress-inducing agents. Dysregulation of MT1X has been implicated in various pathologies, including neurodegenerative diseases, cancer progression, and metal toxicity.
Recombinant MT1X protein is produced using genetic engineering techniques, often expressed in bacterial (e.g., *E. coli*) or mammalian systems. The recombinant form retains the native protein's metal-binding capacity and antioxidant properties, making it a valuable tool for studying metal metabolism, oxidative stress responses, and disease mechanisms. It is widely used in biochemical assays, *in vitro* metal chelation studies, and as a reference standard in diagnostic research.
The production of MT1X recombinant protein involves cloning the MT1X gene into an expression vector, followed by purification via chromatography methods. Quality control includes verifying metal-binding activity, purity (e.g., SDS-PAGE), and structural integrity (e.g., mass spectrometry). Its applications span basic research, drug development, and environmental toxicology, offering insights into cellular defense mechanisms against metal-induced damage and oxidative stress.
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