| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPS22 |
| Uniprot No | P82650 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-360aa |
| 氨基酸序列 | MAPLGTTVLLWSLLRSSPGVERVCFRARIQPWHGGLLQPLPCSFEMGLPRRRFSSEAAESGSPETKKPTFMDEEVQSILTKMTGLNLQKTFKPAIQELKPPTYKLMTQAQLEEATRQAVEAAKVRLKMPPVLEERVPINDVLAEDKILEGTETTKYVFTDISYSIPHRERFIVVREPSGTLRKASWEERDRMIQVYFPKEGRKILTPIIFKEENLRTMYSQDRHVDVLNLCFAQFEPDSTEYIKVHHKTYEDIDKRGKYDLLRSTRYFGGMVWYFVNNKKIDGLLIDQIQRDLIDDATNLVQLYHVLHPDGQSAQGAKDQAAEGINLIKVFAKTEAQKGAYIELTLQTYQEALSRHSAAS |
| 预测分子量 | 68.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MRPS22重组蛋白的3篇参考文献示例(注：文献信息为模拟生成，实际文献需通过学术数据库查询确认)：
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1. **文献名称**: "Functional characterization of MRPS22 mutations in mitochondrial translation deficiency"
**作者**: Smith A, et al.
**摘要**: 本研究通过重组表达人源MRPS22蛋白，发现其突变体导致线粒体翻译缺陷。利用体外重组系统证实突变体影响核糖体小亚基组装，并与复合物I活性下降相关，为线粒体疾病的分子机制提供依据。
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2. **文献名称**: "Structural insights into MRPS22 and its role in mitoribosome assembly"
**作者**: Chen L, et al.
**摘要**: 通过重组表达并纯化MRPS22蛋白，结合冷冻电镜技术解析其在线粒体核糖体小亚基中的三维结构。结果表明，MRPS22通过与MRPS18B等亚基相互作用维持核糖体稳定性，突变可能导致组装障碍。
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3. **文献名称**: "MRPS22 recombinant protein rescues oxidative phosphorylation defects in patient-derived fibroblasts"
**作者**: Wang Y, et al.
**摘要**: 研究构建了野生型MRPS22重组蛋白，并导入携带MRPS22突变的患者成纤维细胞。结果显示，重组蛋白恢复了线粒体氧化磷酸化功能，证明基因治疗策略的潜在应用价值。
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如需具体文献，建议在PubMed或Google Scholar中搜索关键词："MRPS22 recombinant protein" "mitochondrial ribosome assembly" "MRPS22 gene therapy"。
**Background of MRPS22 Recombinant Protein**
Mitochondrial ribosomal protein S22 (MRPS22) is a key component of the mitochondrial ribosome’s small subunit (28S), essential for mitochondrial protein synthesis. Mitochondria, the cellular powerhouses, rely on their own translational machinery to produce subunits of oxidative phosphorylation (OXPHOS) complexes, which generate ATP. Unlike nuclear-encoded ribosomal proteins, MRPS22 is encoded by the nuclear genome, synthesized in the cytoplasm, and imported into mitochondria, where it assembles into functional ribosomes.
Mutations in *MRPS22* have been linked to mitochondrial disorders, such as combined oxidative phosphorylation deficiency, often presenting with symptoms like hypotonia, developmental delay, and lactic acidosis. These findings highlight its critical role in maintaining mitochondrial function and cellular energy homeostasis.
Recombinant MRPS22 protein is produced using heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells) to enable detailed functional and structural studies. Its recombinant form allows researchers to investigate interactions with other ribosomal components, assess the impact of pathogenic mutations, and explore its role in mitochondrial translation regulation. Additionally, it serves as a tool for developing diagnostic assays or therapeutic strategies targeting mitochondrial diseases.
The production of recombinant MRPS22 typically involves cloning the *MRPS22* gene into expression vectors, followed by purification via affinity chromatography. This protein’s availability accelerates research into mitochondrial ribosome assembly dynamics, disease mechanisms, and potential interventions to restore OXPHOS function in genetic or acquired mitochondrial deficiencies.
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