| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL12 |
| Uniprot No | P52815 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-198aa |
| 氨基酸序列 | EALAGAPLDNAPKEYPPKIQQLVQDIASLTLLEISDLNELLKKTLKIQDVGLVPMGGVMSGAVPAAAAQEAVEEDIPIAKERTHFTVRLTEAKPVDKVKLIKEIKNYIQGINLVQAKKLVESLPQEIKANVAKAEAEKIKAALEAVGGTVVLE |
| 预测分子量 | 43.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MRPL12重组蛋白的3篇代表性文献概览：
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1. **文献名称**：*Mitochondrial ribosomal protein L12 interacts with the small GTPase Rab2 and promotes oxidative phosphorylation*
**作者**：Wang Y, et al.
**摘要**：研究利用重组MRPL12蛋白揭示了其与Rab2 GTP酶的相互作用，发现该互作通过调控线粒体膜结构与呼吸链复合体稳定性影响氧化磷酸化功能。
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2. **文献名称**：*Structural insights into the role of MRPL12 in mitochondrial ribosome assembly*
**作者**：Brown A, et al.
**摘要**：通过表达纯化的重组MRPL12蛋白，结合冷冻电镜技术解析其在线粒体核糖体大亚基组装中的构象变化，阐明其对核糖体成熟的关键作用。
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3. **文献名称**：*Recombinant MRPL12 expression rescues mitochondrial translation defects in cellular models*
**作者**：Zhang L, et al.
**摘要**：利用重组MRPL12蛋白在患者来源细胞中进行功能补偿实验，证实其可逆转线粒体翻译缺陷，为相关代谢疾病的治疗提供潜在策略。
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注：以上为模拟示例，实际文献需通过学术数据库检索确认。
**Background of MRPL12 Recombinant Protein**
MRPL12 (Mitochondrial Ribosomal Protein L12) is a key component of the mitochondrial ribosome, specifically the large (39S) subunit, which is essential for mitochondrial protein synthesis. Mitochondria possess their own translation machinery to produce core subunits of the oxidative phosphorylation (OXPHOS) complexes, critical for ATP generation. MRPL12 plays a dual role: it stabilizes the ribosomal structure and facilitates the assembly of functional mitochondrial ribosomes, while also interacting with regulatory factors to coordinate translation with cellular energy demands.
Dysregulation of MRPL12 is linked to mitochondrial dysfunction, contributing to diseases such as neurodegenerative disorders, metabolic syndromes, and cancer. For instance, reduced MRPL12 expression impairs mitochondrial translation, leading to OXPHOS deficiencies and increased reactive oxygen species (ROS), which are implicated in aging and neurodegeneration.
Recombinant MRPL12 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable *in vitro* studies. Its purification typically involves affinity chromatography followed by rigorous quality checks (e.g., SDS-PAGE, Western blot). This recombinant tool allows researchers to investigate MRPL12’s structural interactions within the ribosome, its role in mitochondrial gene expression, and its regulatory mechanisms. Additionally, it aids in screening therapeutic compounds targeting mitochondrial translation defects or exploring MRPL12’s potential as a biomarker.
Studies using recombinant MRPL12 have advanced our understanding of mitochondrial ribosome biogenesis and its crosstalk with nuclear-encoded factors. By providing a controlled, high-purity protein source, recombinant MRPL12 supports both basic research and drug discovery efforts aimed at mitigating mitochondrial dysfunction-related pathologies.
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