| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MFF |
| Uniprot No | Q9GZY8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-342aa |
| 氨基酸序列 | MSKGTSSDTSLGRVSRAAFPSPTAAEMAEISRIQYEMEYTEGISQRMRVPEKLKVAPPNADLEQGFQEGVPNASVIMQVPERIVVAGNNEDVSFSRPADLDLIQSTPFKPLALKTPPRVLTLSERPLDFLDLERPPTTPQNEEIRAVGRLKRERSMSENAVRQNGQLVRNDSLWHRSDSAPRNKISRFQAPISAPEYTVTPSPQQARVCPPHMLPEDGANLSSARGILSLIQSSTRRAYQQILDVLDENRRPVLRGGSAAATSNPHHDNVRYGISNIDTTIEGTSDDLTVVDAASLRRQIIKLNRRLQLLEEENKERAKREMVMYSITVAFWLLNSWLWFRR |
| 预测分子量 | 38,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与MFF(线粒体分裂因子)重组蛋白相关的文献摘要示例(注：文献为虚拟示例，实际研究中请通过学术数据库检索)：
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1. **文献名称**：*Recombinant MFF protein facilitates mitochondrial fission in vitro*
**作者**：Zhang, L. et al.
**摘要**：本研究通过原核表达系统成功纯化了重组人源MFF蛋白，并验证其在体外促进线粒体分裂的功能。实验表明，重组MFF与DRP1蛋白相互作用，增强其在线粒体膜上的聚集能力。
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2. **文献名称**：*Structural analysis of MFF-DRP1 complex by cryo-EM*
**作者**：Tanaka, R. & Yamashita, S.
**摘要**：利用冷冻电镜技术解析了重组MFF蛋白与DRP1的复合物结构，揭示了MFF通过特定结构域招募DRP1至线粒体表面的分子机制，为线粒体动态调控提供结构基础。
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3. **文献名称**：*High-yield production of bioactive MFF in mammalian cells*
**作者**：Chen, X. et al.
**摘要**：开发了一种基于HEK293细胞的哺乳动物表达系统，实现了重组MFF蛋白的高效分泌表达，产物具有天然构象和生物活性，适用于功能研究与药物筛选。
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**提示**：实际文献需通过PubMed、Web of Science等平台检索关键词“MFF recombinant protein”、“Mitochondrial Fission Factor expression”获取。可重点关注线粒体动力学、疾病模型(如神经退行性疾病)相关研究。
**Background of MFF Recombinant Protein**
Mitochondrial Fission Factor (MFF) is a critical regulator of mitochondrial dynamics, primarily involved in mediating mitochondrial fission—a process essential for maintaining mitochondrial health, cellular energy balance, and apoptosis. MFF acts as an adaptor protein that recruits Dynamin-related protein 1 (DRP1) to the mitochondrial outer membrane, facilitating DRP1 oligomerization and subsequent constriction of mitochondria. Dysregulation of MFF is linked to mitochondrial fragmentation, impaired energy metabolism, and neurodegenerative disorders such as Parkinson’s disease.
Recombinant MFF protein is engineered using genetic cloning techniques, where the MFF gene is expressed in heterologous systems like *E. coli* or mammalian cell cultures. This allows large-scale production of purified, functional MFF for research applications. The recombinant protein retains key structural domains, including its C-terminal region critical for DRP1 binding and mitochondrial localization. Post-translational modifications (e.g., phosphorylation) may be preserved in mammalian expression systems, enhancing physiological relevance.
MFF recombinant protein is widely utilized to study mitochondrial fission mechanisms, screen therapeutic compounds targeting mitochondrial dynamics, and model diseases associated with fission defects. It serves as a tool in *in vitro* binding assays, structural studies, and cellular rescue experiments to explore MFF-DRP1 interactions. Additionally, it aids in elucidating how MFF mutations disrupt mitochondrial networks, contributing to neurodegeneration or metabolic disorders.
The development of MFF recombinant protein underscores its importance in advancing mitochondrial biology and therapeutic strategies for mitochondrial-related pathologies. Its applications span basic research, drug discovery, and functional genomics, making it a vital resource in cellular and molecular studies.
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