| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MATN3 |
| Uniprot No | O15232 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-486aa |
| 氨基酸序列 | DPVARPGFRRLETRGPGGSPGRRPSPAAPDGAPASGTSEPGRARGAGVCKSRPLDLVFIIDSSRSVRPLEFTKVKTFVSRIIDTLDIGPADTRVAVVNYASTVKIEFQLQAYTDKQSLKQAVGRITPLSTGTMSGLAIQTAMDEAFTVEAGAREPSSNIPKVAIIVTDGRPQDQVNEVAARAQASGIELYAVGVDRADMASLKMMASEPLEEHVFYVETYGVIEKLSSRFQETFCALDPCVLGTHQCQHVCISDGEGKHHCECSQGYTLNADKKTCSALDRCALNTHGCEHICVNDRSGSYHCECYEGYTLNEDRKTCSAQDKCALGTHGCQHICVNDRTGSHHCECYEGYTLNADKKTCSVRDKCALGSHGCQHICVSDGAASYHCDCYPGYTLNEDKKTCSATEEARRLVSTEDACGCEATLAFQDKVSSYLQRLNTKLDDILEKLKINEYGQIHR |
| 预测分子量 | 57.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MATN3重组蛋白的3-4篇参考文献示例(注：文献为模拟生成，非真实存在)：
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1. **文献名称**: *Recombinant expression and functional characterization of human matrilin-3 in cartilage development*
**作者**: Zhang Y, Chen L, et al.
**摘要**: 研究报道了人源MATN3重组蛋白在哺乳动物细胞中的高效表达与纯化，证实其通过结合Ⅱ型胶原和软骨寡聚基质蛋白(COMP)调控软骨细胞外基质的组装，为研究软骨发育机制提供工具。
2. **文献名称**: *MATN3 mutations alter secretion and extracellular matrix interactions of recombinant protein in vitro*
**作者**: Jackson GC, Mittaz-Crettol L, et al.
**摘要**: 通过构建携带骨软骨发育不良相关突变的MATN3重组蛋白，发现部分突变导致内质网滞留和分泌缺陷，并减弱其与胶原纤维网络的结合能力，提示突变致病的分子机制。
3. **文献名称**: *Matrilin-3 recombinant protein attenuates osteoarthritis progression by modulating TGF-β signaling*
**作者**: Koelling S, Miosge N, et al.
**摘要**: 在小鼠模型中，外源性重组MATN3蛋白通过激活TGF-β/Smad通路促进软骨细胞合成代谢，减缓骨关节炎进展，表明其潜在治疗价值。
4. **文献名称**: *Cryo-EM structure of the MATN3 trimer reveals a role in extracellular matrix elasticity*
**作者**: Wang Q, Xu J, et al.
**摘要**: 利用重组MATN3蛋白解析了其三聚体冷冻电镜结构，发现C端结构域介导的多聚化对维持软骨基质弹性至关重要，为设计靶向药物提供结构依据。
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**注意**：以上文献为示例性质，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实发表的论文。
**Background of MATN3 Recombinant Protein**
Matrilin-3 (MATN3) is a member of the matrilin family, a group of extracellular matrix (ECM) proteins predominantly expressed in cartilage and bone tissues. It plays a critical role in modulating ECM organization, particularly during skeletal development and homeostasis. Structurally, MATN3 contains a von Willebrand factor A (vWA) domain, epidermal growth factor (EGF)-like repeats, and a coiled-coil oligomerization domain, enabling interactions with collagen fibrils and other ECM components. These interactions contribute to cartilage stability, chondrocyte differentiation, and mechanotransduction signaling.
Recombinant MATN3 protein is engineered using heterologous expression systems (e.g., mammalian or bacterial cells) to produce purified, biologically active forms of the protein for research and therapeutic applications. Its production often involves codon optimization, affinity tagging, and refolding steps to ensure proper conformation and functionality. Studies utilizing recombinant MATN3 have shed light on its role in skeletal diseases, such as osteoarthritis and chondrodysplasias, where mutations or dysregulation of MATN3 are linked to abnormal ECM remodeling and cartilage degeneration.
Additionally, recombinant MATN3 serves as a tool to investigate signaling pathways involving integrins and growth factors, which are vital for tissue repair and regeneration. Recent research also explores its potential in biomaterial engineering for cartilage tissue scaffolds. Despite progress, challenges remain in understanding its precise molecular mechanisms and therapeutic targeting. Overall, recombinant MATN3 is a valuable reagent for advancing studies in skeletal biology, disease modeling, and regenerative medicine.
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