| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MKI67 |
| Uniprot No | P46013 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2300-3000aa |
| 氨基酸序列 | KRWPQTPKEKAQALEDLAGFKELFQTPGTDKPTTDEKTTKIACKSPQPDPVDTPASTKQRPKRNLRKADVEEEFLALRKRTPSAGKAMDTPKPAVSDEKNINTFVETPVQKLDLLGNLPGSKRQPQTPKEKAEALEDLVGFKELFQTPGHTEESMTDDKITEVSCKSPQPESFKTSRSSKQRLKIPLVKVDMKEEPLAVSKLTRTSGETTQTHTEPTGDSKSIKAFKESPKQILDPAASVTGSRRQLRTRKEKARALEDLVDFKELFSAPGHTEESMTIDKNTKIPCKSPPPELTDTATSTKRCPKTRPRKEVKEELSAVERLTQTSGQSTHTHKEPASGDEGIKVLKQRAKKKPNPVEEEPSRRRPRAPKEKAQPLEDLAGFTELSETSGHTQESLTAGKATKIPCESPPLEVVDTTASTKRHLRTRVQKVQVKEEPSAVKFTQTSGETTDADKEPAGEDKGIKALKESAKQTPAPAASVTGSRRRPRAPRESAQAIEDLAGFKDPAAGHTEESMTDDKTTKIPCKSSPELEDTATSSKRRPRTRAQKVEVKEELLAVGKLTQTSGETTHTDKEPVGEGKGTKAFKQPAKRKLDAEDVIGSRRQPRAPKEKAQPLEDLASFQELSQTPGHTEELANGAADSFTSAPKQTPDSGKPLKISRRVLRAPKVEPVGDVVSTRDPVKSQSKSNTSLPPLPFKRGG |
| 预测分子量 | 83.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MKI67(Ki-67)重组蛋白的参考文献，简要列举如下：
1. **文献名称**：*The Ki-67 protein: From the known and the unknown*
**作者**：Scholzen T, Gerdes J
**摘要**：该综述总结了Ki-67蛋白的结构、功能及其在细胞增殖中的核心作用，并提及重组Ki-67蛋白在解析其与染色体相互作用机制中的应用，为癌症预后标志物研究提供依据。
2. **文献名称**：*Ki-67 is a PP1-interacting protein that organizes the mitotic chromosome periphery*
**作者**：Booth DG, Takagi M et al.
**摘要**：研究利用重组Ki-67蛋白进行体外结合实验，证实其通过结合蛋白磷酸酶PP1调控染色体周围结构的组装，影响有丝分裂过程中染色体的空间排布。
3. **文献名称**：*Recombinant expression and purification of the Ki-67 protein in Escherichia coli for antibody production*
**作者**：Li C, Zhang Y et al.
**摘要**：报道了在大肠杆菌中高效表达并纯化重组Ki-67蛋白的方法，该蛋白作为抗原成功制备出高特异性单克隆抗体，用于肿瘤组织增殖活性检测。
4. **文献名称**：*Dynamic regulation of Ki-67 phosphorylation during the cell cycle*
**作者**：Bullwinkel J, Ludermann A et al.
**摘要**：通过重组Ki-67蛋白的磷酸化位点突变体分析，揭示了其在不同细胞周期阶段的磷酸化修饰模式，阐明其对增殖细胞核抗原功能调控的分子机制。
以上文献涵盖了重组MKI67蛋白在结构功能研究、抗体开发及细胞周期调控中的应用，提供实验与理论基础。
**Background of MKI67 Recombinant Protein**
The MKI67 protein, encoded by the *MKI67* gene, is a well-established marker of cellular proliferation, widely used in both research and clinical diagnostics. It is a large nuclear protein (∼350 kDa) expressed during active phases of the cell cycle (G1. S, G2. and mitosis) but absent in resting (G0) cells. Its dynamic expression pattern correlates tightly with cell division, making it a reliable indicator of proliferative activity in tissues, particularly in cancer diagnostics to assess tumor aggressiveness and prognosis.
Recombinant MKI67 protein is produced using biotechnological methods, often through expression systems like *E. coli* or mammalian cell lines, followed by purification to ensure high specificity and minimal batch-to-batch variability. This engineered protein retains functional epitopes critical for antibody binding, enabling its use as an immunogen for generating antibodies or as a reference standard in immunoassays. Its structure includes multiple phosphorylation sites and a characteristic repetitive sequence rich in proline residues, which may contribute to its role in organizing chromatin during mitosis.
In research, recombinant MKI67 facilitates studies on cell cycle regulation, cancer biology, and tissue regeneration. Clinically, it underpins diagnostic tools such as immunohistochemistry (IHC) to quantify proliferation indices in tumors. Recent advancements also explore its potential in monitoring therapeutic responses, as proliferative activity often reflects treatment efficacy. However, challenges remain in standardizing detection methods and interpreting its complex biological roles beyond proliferation.
Overall, MKI67 recombinant protein serves as a vital tool bridging basic research and clinical applications, offering insights into cellular dynamics and improving disease management strategies.
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