| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL19 |
| Uniprot No | P49406 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-292aa |
| 氨基酸序列 | MAACIAAGHWAAMGLGRSFQAARTLLPPPASIACRVHAGPVRQQSTGPSEPGAFQPPPKPVIVDKHRPVEPERRFLSPEFIPRRGRTDPLKFQIERKDMLERRKVLHIPEFYVGSILRVTTADPYASGKISQFLGICIQRSGRGLGATFILRNVIEGQGVEICFELYNPRVQEIQVVKLEKRLDDSLLYLRDALPEYSTFDVNMKPVVQEPNQKVPVNELKVKMKPKPWSKRWERPNFNIKGIRFDLCLTEQQMKEAQKWNQPWLEFDMMREYDTSKIEAAIWKEIEASKRS |
| 预测分子量 | 60.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MRPL19重组蛋白的3篇示例文献(内容为示例性概括，非真实文献)：
1. **《Structural characterization of human MRPL19 recombinant protein and its role in mitochondrial ribosome assembly》**
- **作者**: Chen L, et al.
- **摘要**: 通过大肠杆菌表达系统纯化重组MRPL19蛋白，结合冷冻电镜分析其在线粒体核糖体大亚基组装中的构象变化，揭示其与16S rRNA的相互作用位点。
2. **《MRPL19 overexpression promotes tumor cell proliferation via mitochondrial translation enhancement in colorectal cancer》**
- **作者**: Wang Y, et al.
- **摘要**: 研究重组MRPL19蛋白在结直肠癌细胞中的功能，发现其过表达通过激活线粒体蛋白翻译通路促进肿瘤生长，为靶向治疗提供潜在分子标记。
3. **《A novel method for high-yield purification of recombinant MRPL19 and its immunogenicity analysis》**
- **作者**: Gupta S, et al.
- **摘要**: 开发基于昆虫细胞表达系统的MRPL19重组蛋白高效纯化方法，并验证其在动物模型中诱导特异性抗体的免疫原性，为相关自身免疫疾病研究奠定基础。
(注：以上文献为虚拟示例，实际研究需通过PubMed、Web of Science等平台检索确认。)
MRPL19 (Mitochondrial Ribosomal Protein L19) is a key component of the mitochondrial ribosome large subunit, playing an essential role in mitochondrial protein synthesis. Mitochondria, the cellular powerhouses, rely on their own translation machinery to produce subunits of oxidative phosphorylation complexes critical for ATP generation. MRPL19 is encoded by nuclear DNA, synthesized in the cytoplasm, and subsequently imported into mitochondria, where it integrates into the 39S large ribosomal subunit. This protein contributes to ribosome assembly, stability, and translational fidelity, ensuring proper synthesis of mitochondrially encoded proteins like ND1-6. CYTB, and ATP6/8.
Recombinant MRPL19 refers to the protein produced artificially using recombinant DNA technology, typically expressed in bacterial (e.g., E. coli) or eukaryotic systems (e.g., HEK293 cells). The recombinant version retains the functional domains of the native protein while enabling controlled production for research applications. Its expression often includes purification tags (e.g., His-tag) for efficient isolation.
Studies involving recombinant MRPL19 focus on mitochondrial dysfunction linked to diseases such as cancer, neurodegenerative disorders, and metabolic syndromes. It serves as a tool to investigate ribosome biogenesis defects, mitochondrial translation impairments, and cellular energy metabolism. Additionally, it aids in developing diagnostic biomarkers or therapeutic targets, particularly in cancers where MRPL19 overexpression correlates with poor prognosis. Researchers also utilize it to generate antibodies for detecting endogenous protein levels or to study interactions within mitochondrial ribosome complexes. Its recombinant form provides a standardized material for structural studies, enzyme assays, and drug screening platforms targeting mitochondrial diseases.
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