| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL42 |
| Uniprot No | Q9Y6G3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 33-142aa |
| 氨基酸序列 | KSTYSPLPDDYNCNVELALTSDGRTIVCYHPSVDIPYEHTKPIPRPDPVHNNEETHDQVLKTRLEEKVEHLEEGPMIEQLSKMFFTTKHRWYPHGRYHRCRKNLNPPKDR |
| 预测分子量 | 40.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MRPL42重组蛋白的模拟参考文献示例(仅供参考，实际文献需通过学术数据库验证)：
---
1. **"Structural characterization of MRPL42 in the mitochondrial ribosome assembly"**
*Authors: Brown A, et al. (2020)*
**摘要**：本研究利用重组表达的MRPL42蛋白，通过冷冻电镜技术解析其在线粒体核糖体大亚基中的三维结构，揭示了其与邻近蛋白的相互作用界面，为线粒体翻译机制提供结构基础。
2. **"MRPL42 overexpression promotes tumorigenesis via mitochondrial metabolic reprogramming"**
*Authors: Smith J, et al. (2018)*
**摘要**：通过重组MRPL42蛋白的体外功能实验，发现其过表达可增强线粒体氧化磷酸化活性，促进癌细胞增殖，提示其作为潜在癌症治疗靶点的可能性。
3. **"Recombinant MRPL42 expression and its role in apoptosis regulation"**
*Authors: Zhang Y, et al. (2021)*
**摘要**：构建了重组MRPL42真核表达系统，证实其通过调控Bcl-2家族蛋白活性影响线粒体膜通透性，进而参与细胞凋亡信号通路。
4. **"Optimization of MRPL42 recombinant protein production in E. coli"**
*Authors: Lee S, et al. (2019)*
**摘要**：系统优化了MRPL42在大肠杆菌中的重组表达条件，包括诱导温度、IPTG浓度等，获得高纯度蛋白用于后续抗体开发及功能研究。
---
**提示**：以上为模拟文献，实际研究中请通过PubMed、Google Scholar等平台搜索真实文献(关键词：MRPL42. mitochondrial ribosome, recombinant protein)。
MRPL42 (Mitochondrial Ribosomal Protein L42) is a key component of the mitochondrial ribosome large subunit (mt-LSU), essential for mitochondrial protein synthesis. Mitochondria, the energy-producing organelles, rely on their own translation machinery to synthesize core subunits of oxidative phosphorylation (OXPHOS) complexes, which are critical for ATP production. MRPL42. encoded by nuclear DNA, is imported into mitochondria and assembled into the 39S mt-LSU, facilitating the translation of mitochondrial DNA (mtDNA)-encoded mRNAs.
Recombinant MRPL42 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cell cultures) to study its structural and functional roles. Its production enables detailed investigations into mitochondrial ribosome assembly, translational regulation, and interactions with other ribosomal proteins or translation factors. Dysregulation of MRPL42 has been linked to mitochondrial disorders, such as encephalomyopathies, and cancers, where altered mitochondrial metabolism supports tumor progression. Recombinant MRPL42 also serves as a tool for developing diagnostic assays or therapeutic strategies targeting mitochondrial dysfunction.
Structural studies using recombinant MRPL42. combined with cryo-EM or X-ray crystallography, have advanced our understanding of mitochondrial translation mechanisms. Additionally, it aids in exploring post-translational modifications or mutations affecting ribosome stability. Research on MRPL42 highlights its dual role in maintaining mitochondrial proteostasis and cellular energy balance, emphasizing its relevance in both basic science and clinical applications.
×
