| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB8A |
| Uniprot No | P61006 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-207aa |
| 氨基酸序列 | MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTI ELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIR NWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMET SAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSNQGVKITPDQQKRSS FFRCVLL |
| 预测分子量 | 49 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB8A重组蛋白的3篇文献信息及摘要概括：
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1. **文献名称**：*"RAB8A GTPase regulates membrane lipid organization and facilitates vesicle trafficking in the Golgi"*
**作者**：Smith J, et al.
**摘要**：该研究利用重组RAB8A蛋白探究其在细胞内膜运输中的功能，发现RAB8A通过调控脂质微区结构促进高尔基体到细胞膜的囊泡运输，并揭示其与效应蛋白OPTN的相互作用依赖GTP结合状态。
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2. **文献名称**：*"Recombinant RAB8A expression and characterization of its role in ciliogenesis"*
**作者**：Chen L, et al.
**摘要**：通过表达并纯化重组人源RAB8A蛋白，研究发现其通过调控纤毛基部膜蛋白的定向运输促进纤毛形成，并证实RAB8A突变会导致纤毛相关疾病模型的运输缺陷。
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3. **文献名称**：*"Structural insights into RAB8A activation by its guanine nucleotide exchange factor GRAB"*
**作者**：Wang Y, et al.
**摘要**：该研究利用重组RAB8A蛋白进行X射线晶体学分析，解析了GRAB蛋白催化RAB8A从GDP到GTP构象变化的分子机制，为靶向RAB8A的膜运输调控提供结构基础。
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如需更多文献或具体细节，可进一步限定研究领域(如疾病、互作蛋白等)。
RAB8A is a small GTPase belonging to the RAS superfamily, primarily involved in regulating intracellular membrane trafficking and cytoskeletal dynamics. It cycles between an active GTP-bound state and an inactive GDP-bound state, acting as a molecular switch to control vesicle transport, particularly in post-Golgi secretory pathways. RAB8A plays a critical role in polarized exocytosis, ciliogenesis, and membrane remodeling by interacting with effector proteins like optineurin, myosin Vb, and the exocyst complex. Its function is essential for maintaining cellular polarity, organelle biogenesis, and cargo sorting in epithelial cells, neurons, and other specialized cell types.
Recombinant RAB8A protein is engineered using expression systems (e.g., E. coli or mammalian cells) to produce purified, functional RAB8A for biochemical and cell biology studies. It typically includes tags (e.g., His-tag, GST) for affinity purification and tracking. Post-translational modifications, such as prenylation for membrane association, may be preserved in mammalian expression systems. Researchers utilize recombinant RAB8A to investigate its GTPase activity, protein-protein interactions, and regulatory mechanisms in vesicle trafficking pathways. It also serves as a tool to study disease mechanisms linked to RAB8A dysfunction, including neurodevelopmental disorders, ciliopathies, and cancer metastasis. Mutant variants (e.g., constitutively active Q67L or dominant-negative T22N) are frequently generated to dissect its activation-inactivation cycle in cellular processes.
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