| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EIF4EBP3 |
| Uniprot No | O60516 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-100aa |
| 氨基酸序列 | MSTSTSCPIPGGRDQLPDCYSTTPGGTLYATTPGGTRIIYDRKFLLECKNSPIARTPPCCLPQIPGVTTPPTAPLSKLEELKEQETEEEIPDDAQFEMDI |
| 预测分子量 | 26.9kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **EIF4EBP3重组蛋白** 的3篇参考文献示例(内容为虚构，供参考)：
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1. **文献名称**: *"Functional characterization of recombinant EIF4EBP3 and its role in mTOR signaling regulation"*
**作者**: Zhang L, et al.
**摘要**: 本研究通过大肠杆菌表达系统成功制备了重组人源EIF4EBP3蛋白，并验证其与mTORC1下游靶点eIF4E的结合能力。实验表明，EIF4EBP3通过抑制eIF4E介导的mRNA帽依赖性翻译，调控细胞增殖和代谢。
2. **文献名称**: *"Structural insights into EIF4EBP3 phosphorylation and interaction with eIF4E"*
**作者**: Kim S, et al.
**摘要**: 利用X射线晶体学解析了重组EIF4EBP3蛋白的磷酸化结构，揭示了其与eIF4E结合的关键结构域。磷酸化修饰显著增强EIF4EBP3对eIF4E的抑制作用，提示其在癌症治疗中的潜在靶点价值。
3. **文献名称**: *"EIF4EBP3 recombinant protein suppresses tumor growth in a xenograft model of breast cancer"*
**作者**: Patel R, et al.
**摘要**: 通过体外和体内实验证明，重组EIF4EBP3蛋白可有效抑制乳腺癌细胞的侵袭和迁移能力，其机制可能与阻断mTOR通路并诱导细胞周期停滞相关。
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*注：以上文献为示例，实际研究中需通过PubMed或Google Scholar检索真实文献。*
**Background of EIF4EBP3 Recombinant Protein**
EIF4EBP3 (Eukaryotic Translation Initiation Factor 4E-Binding Protein 3) is a member of the EIF4EBP family, which regulates cap-dependent translation initiation by interacting with eukaryotic initiation factor 4E (eIF4E). This interaction inhibits the assembly of the eIF4F complex, a critical mediator of mRNA translation. While EIF4EBP1 and EIF4EBP2 are well-studied for their roles in cellular stress responses, growth regulation, and cancer progression, EIF4EBP3 remains less characterized. It shares structural homology with its family members, including conserved eIF4E-binding motifs, but exhibits distinct expression patterns and regulatory mechanisms. EIF4EBP3 is thought to fine-tune translational control under specific physiological or pathological conditions, potentially modulating cell proliferation, survival, or differentiation.
Recombinant EIF4EBP3 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Its production often involves cloning the EIF4EBP3 gene into expression vectors, followed by purification via affinity tags (e.g., His-tag). Researchers utilize this recombinant protein to investigate its binding affinity to eIF4E, its phosphorylation dynamics (e.g., in response to mTOR signaling), and its role in downstream pathways like the PI3K/AKT/mTOR axis.
Interest in EIF4EBP3 has grown due to its potential implications in diseases such as cancer, where dysregulated translation contributes to tumorigenesis. Studies suggest it may act as a tumor suppressor or context-dependent modulator, though conflicting findings highlight the need for further research. Additionally, recombinant EIF4EBP3 serves as a tool for screening therapeutic agents targeting translation initiation, offering avenues for cancer therapy. Challenges in studying EIF4EBP3 include its low endogenous expression and functional redundancy with other EIF4EBPs. Nonetheless, its recombinant form provides a controlled means to dissect its unique contributions to cellular and disease mechanisms.
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