| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL29 |
| Uniprot No | P47914 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-159aa |
| 氨基酸序列 | AKSKNHTTHNQSRKWHRNGIKKPRSQRYESLKGVDPKFLRNMRFAKKHNKKGLKKMQANNAKAMSARAEAIKALVKPKEVKPKIPKGVSRKLDRLAYIAHPKLGKRARARIAKGLRLCRPKAKAKAKAKDQTKAQAAAPASVPAQAPKRTQAPTKASE |
| 预测分子量 | 44.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于RPL29重组蛋白的参考文献(仅供参考，非真实文献)：
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1. **标题**: *"Recombinant RPL29 promotes tumor cell proliferation via Wnt/β-catenin signaling"*
**作者**: Zhang, Y. et al.
**摘要**: 研究通过重组表达RPL29蛋白，发现其通过激活Wnt/β-catenin通路增强结直肠癌细胞增殖，提示其在肿瘤进展中的潜在作用。
2. **标题**: *"Expression and structural characterization of human RPL29 in E. coli"*
**作者**: Smith, J.R. & Lee, H.
**摘要**: 报道了利用大肠杆菌系统高效表达并纯化重组RPL29.通过X射线晶体学解析其三维结构，为功能研究奠定基础。
3. **标题**: *"RPL29 regulates embryonic stem cell differentiation through ribosome biogenesis"*
**作者**: García-Sánchez, A. et al.
**摘要**: 发现重组RPL29通过调控核糖体合成影响胚胎干细胞的自我更新与分化，揭示其发育生物学中的新机制。
4. **标题**: *"RPL29 interacts with p53: Implications for cancer therapy"*
**作者**: Chen, L. et al.
**摘要**: 重组RPL29被证实与p53蛋白直接互作，可能通过调控p53稳定性影响乳腺癌细胞化疗敏感性。
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**注意**：以上为模拟内容，实际文献需通过PubMed、Google Scholar等平台检索关键词(如“RPL29 recombinant”“RPL29 function”)。建议结合具体研究方向筛选最新高质量论文。
**Background of Recombinant RPL29 Protein**
Ribosomal protein L29 (RPL29), a component of the 60S ribosomal subunit, plays a critical role in protein synthesis by facilitating ribosome assembly and translation. Beyond its canonical function, RPL29 has been implicated in diverse cellular processes, including cell proliferation, differentiation, and extracellular matrix interactions. Studies suggest its involvement in developmental regulation, particularly in tissues like the intestine and mammary glands, where it may modulate epithelial-mesenchymal crosstalk.
Recombinant RPL29 protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or mammalian systems to ensure proper folding and post-translational modifications. This engineered protein retains the functional domains of native RPL29. enabling researchers to study its structure-function relationships, interactions with RNA or other proteins, and regulatory roles in diseases. For instance, dysregulation of RPL29 has been linked to cancers, such as colorectal and breast cancer, where its overexpression may promote tumor progression.
In research, recombinant RPL29 serves as a tool to investigate ribosome biogenesis defects, cellular stress responses, and its potential as a biomarker or therapeutic target. Its applications extend to *in vitro* binding assays, antibody production, and mechanistic studies in developmental biology. Additionally, RPL29's role in mediating cell adhesion through interactions with integrins highlights its relevance in tissue engineering and regenerative medicine.
Overall, recombinant RPL29 provides a versatile platform for dissecting both ribosomal and extra-ribosomal functions of this protein, bridging molecular biology with translational medicine.
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