| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ELAVL2 |
| Uniprot No | Q12926-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-346aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMETQLSN GPTCNNTANG PTTINNNCSS PVDSGNTEDS KTNLIVNYLP QNMTQEELKS LFGSIGEIES CKLVRDKITG QSLGYGFVNY IDPKDAEKAI NTLNGLRLQT KTIKVSYARP SSASIRDANL YVSGLPKTMT QKELEQLFSQ YGRIITSRIL VDQVTGISRG VGFIRFDKRI EAEEAIKGLN GQKPPGATEP ITVKFANNPS QKTNQAILSQ LYQSPNRRYP GPLAQQAQRF RFSPMTIDGM TSLAGINIPG HPGTGWCIFV YNLAPDADES ILWQMFGPFG AVTNVKVIRD FNTNKCKGFG FVTMTNYDEA AMAIASLNGY RLGDRVLQVS FKTNKTHKA |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ELAVL2重组蛋白的3篇参考文献示例，涵盖其功能、结构及作用机制：
---
1. **文献名称**：*Molecular characterization and RNA-binding properties of recombinant ELAVL2 protein*
**作者**：Smith A, Johnson B, et al.
**摘要**：本研究在大肠杆菌中成功表达并纯化了重组ELAVL2蛋白，通过电泳迁移率变动分析(EMSA)验证其与神经元特异性mRNA(如GAP-43)的结合能力，揭示了ELAVL2在调节突触可塑性中的潜在作用。
2. **文献名称**：*Structural insights into ELAVL2 RNA recognition motifs*
**作者**：Zhang Y, Wang L, et al.
**摘要**：利用重组ELAVL2蛋白的X射线晶体学分析，解析了其RNA结合结构域的三维结构，发现其通过保守的芳香族残基与靶RNA的AU富集区结合，为设计靶向ELAVL2的分子提供了结构基础。
3. **文献名称**：*ELAVL2/HuB stabilizes mRNA during neurodevelopment through dynamic cytoplasmic shuttling*
**作者**：Lee C, Kim H, et al.
**摘要**：通过体外重组ELAVL2蛋白实验，证实其通过结合β-actin等mRNA的3'UTR增强稳定性，并依赖磷酸化修饰调节胞质定位，从而促进神经元分化及轴突生长。
---
*注：以上文献为示例，实际引用需以真实发表的论文为准。建议通过PubMed或Google Scholar以关键词“ELAVL2 recombinant”“ELAVL2 RNA-binding”进一步检索。*
**Background of ELAVL2 Recombinant Protein**
ELAVL2 (Embryonic Lethal, Abnormal Vision-like 2), also known as HuB, is a member of the ELAV/Hu family of RNA-binding proteins. This family, including ELAVL1 (HuR), ELAVL3 (HuC), and ELAVL4 (HuD), plays critical roles in post-transcriptional regulation by binding to AU-rich elements (AREs) in the 3' untranslated regions (UTRs) of target mRNAs. ELAVL2 is particularly notable for its neuron-specific expression and involvement in neuronal development, plasticity, and maintenance. It stabilizes mRNAs, enhances their translation, and regulates splicing, thereby influencing processes like axonal growth, synaptic function, and cellular responses to stress.
Structurally, ELAVL2 contains three RNA recognition motifs (RRMs) that mediate RNA binding and a hinge region facilitating protein-protein interactions. Dysregulation of ELAVL2 has been linked to neurological disorders, including neurodevelopmental defects, neurodegenerative diseases, and brain tumors. Its role in cancer is context-dependent, acting as either an oncogene or tumor suppressor depending on cellular conditions.
Recombinant ELAVL2 protein is produced using expression systems such as *E. coli* or mammalian cells, often fused with tags (e.g., His, GST) for purification. It serves as a vital tool for studying RNA-protein interactions, neuronal differentiation mechanisms, and disease pathways. Researchers utilize it in *in vitro* assays (e.g., electrophoretic mobility shift assays, RNA immunoprecipitation) and cell-based studies to decode its regulatory networks.
Overall, ELAVL2 recombinant protein provides a foundation for exploring post-transcriptional control in neurobiology and disease, offering potential therapeutic targets for conditions involving RNA metabolism dysregulation.
×
