| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC4A1 |
| Uniprot No | P02730 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-403aa |
| 氨基酸序列 | MEELQDDYEDMMEENLEQEEYEDPDIPESQMEEPAAHDTEATATDYHTTSHPGTHKVYVELQELVMDEKNQELRWMEAARWVQLEENLGENGAWGRPHLSHLTFWSLLELRRVFTKGTVLLDLQETSLAGVANQLLDRFIFEDQIRPQDREELLRALLLKHSHAGELEALGGVKPAVLTRSGDPSQPLLPQHSSLETQLFCEQGDGGTEGHSPSGILEKIPPDSEATLVLVGRADFLEQPVLGFVRLQEAAELEAVELPVPIRFLFVLLGPEAPHIDYTQLGRAAATLMSERVFRIDAYMAQSRGELLHSLEGFLDCSLVLPPTDAPSEQALLSLVPVQRELLRRRYQSSPAKPDSSFYKGLDLNGGPDDPLQQTGQLFGGLVRDIRRRYPYYLSDITDAFSP |
| 预测分子量 | 65.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural basis of anion exchange in human erythrocytes by the SLC4A1 protein"** - Zhang, X., et al.
*摘要*：本研究解析了人源SLC4A1重组蛋白的晶体结构，揭示了其跨膜结构域中氯离子和碳酸氢盐的转运机制，阐明了pH依赖性构象变化的分子基础。
2. **"Functional characterization of SLC4A1 mutations associated with hereditary spherocytosis using recombinant protein models"** - Bruce, L.J., et al.
*摘要*：通过重组表达SLC4A1突变体蛋白，分析其离子转运功能及细胞膜定位缺陷，揭示了遗传性球形红细胞增多症相关突变的致病机制。
3. **"Recombinant SLC4A1 anion exchanger dynamics studied by single-molecule fluorescence microscopy"** - Fuster, D.G., & Alper, S.L.
*摘要*：利用荧光标记的重组SLC4A1蛋白，实时观察其在细胞膜上的动态分布及离子转运活性，提出其与细胞骨架相互作用的调控模型。
4. **"Expression and purification of the human SLC4A1 membrane domain for drug interaction studies"** - Fujinaga, J., & Tang, X.
*摘要*：开发了一种高效重组表达系统，用于纯化SLC4A1跨膜结构域蛋白，并筛选出潜在的小分子抑制剂，为治疗溶血性贫血提供新策略。
(注：以上文献信息为示例性质，实际引用需核实具体论文。)
The SLC4A1 gene encodes anion exchanger 1 (AE1), a transmembrane protein critical for maintaining pH and ion balance in erythrocytes and renal tissues. Also known as Band 3 protein in red blood cells, AE1 facilitates the exchange of chloride (Cl⁻) and bicarbonate (HCO₃⁻) across cell membranes, supporting CO₂ transport and acid-base homeostasis. In the kidney, a shorter isoform (kAE1) mediates bicarbonate reabsorption in α-intercalated cells. Dysfunctional AE1 is linked to hereditary disorders, including hereditary spherocytosis (defective erythrocyte stability) and distal renal tubular acidosis (impaired urinary acidification), often caused by mutations affecting protein trafficking or ion transport.
Recombinant SLC4A1 proteins are engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to study AE1's structure-function relationships, disease mechanisms, and therapeutic targeting. These proteins enable detailed biochemical analyses, such as mapping ion-binding sites, characterizing pathogenic mutations, or screening small molecules that modulate transport activity. Crystallography and cryo-EM studies using recombinant AE1 fragments have revealed insights into its dimeric structure, membrane topology, and interaction partners (e.g., hemoglobin or cytoskeletal proteins). Researchers also employ recombinant AE1 to model cellular defects in disease or test gene therapy strategies. Its role in malaria parasite invasion (via Band 3 interactions) further expands its relevance to infectious disease research. By providing a controlled, scalable source of functional protein, SLC4A1 recombinant tools advance both basic science and translational applications in hematology, nephrology, and beyond.
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