| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADRM1 |
| Uniprot No | Q16186 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-407aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMTTSGALFPSLVPGSRGASNKYLVEF RAGKMSLKGTTVTPDKRKGLVYIQQTDDSLIHFCWKDRTSGNVEDDLIIF PDDCEFKRVPQCPSGRVYVLKFKAGSKRLFFWMQEPKTDQDEEHCRKVNE YLNNPPMPGALGASGSSGHELSALGGEGGLQSLLGNMSHSQLMQLIGPAG LGGLGGLGALTGPGLASLLGSSGPPGSSSSSSSRSQSAAVTPSSTTSSTR ATPAPSAPAAASATSPSPAPSSGNGASTAASPTQPIQLSDLQSILATMNV PAGPAGGQQVDLASVLTPEIMAPILANADVQERLLPYLPSGESLPQTADE IQNTLTSPQFQQALGMFSAALASGQLGPLMCQFGLPAEAVEAANKGDVEA FAKAMQNNAKPEQKEGDTKDKKDEEEDMSLD |
| 预测分子量 | 45 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADRM1重组蛋白的3篇参考文献概览：
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1. **文献名称**: "ADRM1/RPN13: A protease-associated domain protein with roles in cancer and proteasome assembly"
**作者**: Husnjak K, et al.
**摘要**: 该研究解析了ADRM1作为26S蛋白酶体的泛素受体功能，通过重组ADRM1蛋白实验证明其C端结构域与蛋白酶体亚基的相互作用，并揭示了其在癌症中过表达与肿瘤进展的关联。
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2. **文献名称**: "Structural basis for the recognition of ubiquitin by the proteasome subunit ADRM1"
**作者**: Shi Y, et al.
**摘要**: 通过X射线晶体学分析重组ADRM1蛋白的泛素结合结构域，阐明了其与泛素链特异性结合的分子机制，为靶向ADRM1的癌症治疗策略提供结构基础。
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3. **文献名称**: "ADRM1 interacts with USP14 and promotes cancer cell proliferation via mTOR signaling"
**作者**: Chen X, et al.
**摘要**: 研究利用重组ADRM1蛋白进行体外结合实验，证实其与去泛素化酶USP14的互作，并发现该互作通过激活mTOR通路促进肿瘤细胞增殖，提示ADRM1作为潜在治疗靶点。
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注：以上文献为示例性概括，实际引用时请核对原文准确性及发表年份。ADRM1亦被称为RPN13.建议结合关键词“ADRM1/RPN13 recombinant”进一步检索。
ADRM1 (Adhesion Regulating Molecule 1), also known as PSMD4 or RPN13. is a multifunctional protein primarily recognized for its role in the ubiquitin-proteasome system (UPS). As a key component of the 19S regulatory particle of the 26S proteasome, ADRM1 acts as a ubiquitin receptor, facilitating the recognition and degradation of polyubiquitinated proteins, a critical process in maintaining cellular protein homeostasis. Its structure includes ubiquitin-interacting motifs (UIMs) that bind ubiquitin chains, enabling substrate targeting for proteasomal degradation. Beyond its proteasomal role, ADRM1 participates in non-proteolytic functions, such as regulating cell adhesion, signal transduction, and DNA repair pathways.
Recombinant ADRM1 protein is engineered using expression systems like *E. coli* or mammalian cells, allowing researchers to study its biochemical properties, interactions, and mechanisms in vitro. This tool has become vital for investigating UPS dysregulation linked to diseases, including cancer and neurodegenerative disorders. Overexpression of ADRM1 has been observed in various cancers, correlating with poor prognosis and chemoresistance, positioning it as a potential therapeutic target. Researchers use recombinant ADRM1 to screen for inhibitors that could block its ubiquitin-binding activity or disrupt proteasome assembly, offering avenues for drug development. Additionally, it aids in structural studies to map binding interfaces or mutations affecting proteasome function. Despite its established roles, ongoing research seeks to clarify ADRM1's non-canonical functions and regulatory networks, highlighting its complexity in cellular physiology and pathology. The recombinant protein thus serves as a cornerstone for both basic research and translational applications in targeted therapy development.
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