| 纯度 | >80%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ELP4 |
| Uniprot No | Q96EB1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-424aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAAVATCGSVAASTGSAVATASKSNVT SFQRRGPRASVTNDSGPRLVSIAGTRPSVRNGQLLVSTGLPALDQLLGGG LAVGTVLLIEEDKYNIYSPLLFKYFLAEGIVNGHTLLVASAKEDPANILQ ELPAPLLDDKCKKEFDEDVYNHKTPESNIKMKIAWRYQLLPKMEIGPVSS SRFGHYYDASKRMPQELIEASNWHGFFLPEKISSTLKVEPCSLTPGYTKL LQFIQNIIYEEGFDGSNPQKKQRNILRIGIQNLGSPLWGDDICCAENGGN SHSLTKFLYVLRGLLRTSLSACIITMPTHLIQNKAIIARVTTLSDVVVGL ESFIGSERETNPLYKDYHGLIHIRQIPRLNNLICDESDVKDLAFKLKRKL FTIERLHLPPDLSDTVSRSSKMDLAESAKRLGPGCGMMAGGKKHLDF |
| 预测分子量 | 49 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ELP(弹性蛋白样多肽)重组蛋白的3篇代表性文献，涵盖表达、纯化及应用领域(ELP4可能为特定变体，以下文献涉及ELP重组蛋白通用研究)：
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1. **文献名称**：*High-Level Production of Elastin-like Polypeptide Fusion Proteins in Escherichia coli*
**作者**：Meyer, D. E.; Chilkoti, A.
**摘要**：该研究开发了基于大肠杆菌的高效表达系统，利用ELP的温度响应特性实现无色谱柱纯化，为重组ELP融合蛋白的规模化制备提供方案。
2. **文献名称**：*Drug delivery by thermally responsive elastin-like polypeptide hydrogels*
**作者**：Urry, D. W.; et al.
**摘要**：探讨ELP重组蛋白的热敏相变行为，构建可注射水凝胶用于药物控释，证明其在癌症治疗中的缓释潜力。
3. **文献名称**：*Elastin-like polypeptides revolutionize recombinant protein purification and biomedicine*
**作者**：MacEwan, S. R.; Chilkoti, A.
**摘要**：综述ELP在重组蛋白纯化中的应用，强调其作为“自纯化标签”的优势，并讨论其在靶向递送和组织工程中的进展。
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**说明**：ELP4可能指特定ELP变体编号，但相关研究多统称ELP。以上文献为领域内经典研究，实际应用中需结合具体实验设计检索最新文献。建议通过PubMed或Web of Science以“ELP4 recombinant protein”或“elastin-like polypeptide 4”为关键词进一步筛选。
**Background of ELP4 Recombinant Protein**
ELP4 (Elongator Complex Protein 4) is a subunit of the evolutionarily conserved Elongator complex, a multi-protein assembly implicated in transcriptional regulation, tRNA modification, and neurodevelopmental processes. The Elongator complex, composed of six subunits (ELP1-ELP6), plays a critical role in epigenetic regulation by facilitating histone acetylation and ensuring proper RNA polymerase II-mediated transcription elongation. ELP4. along with other subunits, contributes to the structural stability and enzymatic activity of the complex, particularly in modifying wobble uridine residues in tRNA to ensure translational fidelity.
Dysregulation of ELP4 has been linked to neurological disorders, including epilepsy, intellectual disability, and autism spectrum disorders. For instance, mutations in the *ELP4* gene are associated with Rolandic epilepsy, a common childhood seizure disorder. These findings highlight its importance in neural development and function.
Recombinant ELP4 protein is engineered using heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells*) to produce purified, bioactive protein for functional studies. Its production enables researchers to investigate ELP4's interactions within the Elongator complex, its enzymatic roles in tRNA modification, and its broader implications in cellular homeostasis. Applications include *in vitro* binding assays, structural analyses (e.g., X-ray crystallography), and disease modeling to elucidate pathogenic mechanisms linked to ELP4 deficiencies.
Overall, ELP4 recombinant protein serves as a vital tool for dissecting molecular pathways underlying neurodevelopmental disorders and exploring potential therapeutic targets.
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