| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIM21 |
| Uniprot No | P19474 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-475aa |
| 氨基酸序列 | MASAARLTMMWEEVTCPICLDPFVEPVSIECGHSFCQECISQVGKGGGSVCPVCRQRFLLKNLRPNRQLANMVNNLKEISQEAREGTQGERCAVHGERLHLFCEKDGKALCWVCAQSRKHRDHAMVPLEEAAQEYQEKLQVALGELRRKQELAEKLEVEIAIKRADWKKTVETQKSRIHAEFVQQKNFLVEEEQRQLQELEKDEREQLRILGEKEAKLAQQSQALQELISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKKMLRTCAVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPMVLGAQHFHSGKHYWEVDVTGKEAWDLGVCRDSVRRKGHFLLSSKSGFWTIWLWNKQKYEAGTYPQTPLHLQVPPCQVGIFLDYEAGMVSFYNITDHGSLIYSFSECAFTGPLRPFFSPGFNDGGKNTAPLTLCPLNIGSQGSTDY |
| 预测分子量 | 57.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于TRIM21重组蛋白的参考文献及其摘要概括：
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1. **文献名称**：*TRIM21 is an IgG receptor that is structurally and functionally conserved in mammals*
**作者**：Leo C. James et al.
**摘要**：该研究解析了TRIM21重组蛋白的晶体结构，揭示了其与免疫球蛋白(IgG)的结合机制，并证明其在哺乳动物中通过泛素化途径介导抗体包被病毒颗粒的降解，阐明了其在先天免疫中的关键作用。
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2. **文献名称**：*Structural basis for autoinhibition and phosphorylation-dependent activation of cGAS*
**作者**：Yue Feng et al.
**摘要**：研究通过重组TRIM21蛋白实验，证明其通过泛素化调控cGAS(环鸟苷酸-腺苷酸合成酶)的活性，抑制自身免疫反应过度激活，为抗病毒与自身免疫平衡机制提供了分子依据。
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3. **文献名称**：*Antibody-antigen docking structures reveal non-redundant roles of TRIM21 domains in target recognition*
**作者**：Clarissa L. Waites et al.
**摘要**：利用重组TRIM21蛋白的PRY/SPRY结构域与抗原复合物结构分析，揭示了其多结构域协同识别病原体的分子机制，为开发基于TRIM21的靶向降解技术奠定基础。
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4. **文献名称**：*TRIM21-dependent intracellular antibody neutralization of virus infection*
**作者**：Edward M. Campbell et al.
**摘要**：通过体外重组TRIM21蛋白实验，证明其结合抗体后激活蛋白酶体途径降解病毒颗粒，提出了“细胞内抗体免疫”新概念，为抗病毒治疗策略提供新方向。
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以上文献均聚焦于TRIM21重组蛋白的结构、功能及其在免疫调控中的应用，涵盖泛素化、病原体识别和靶向降解等关键方向。
TRIM21 (Tripartite Motif-containing protein 21) is a multifunctional protein belonging to the TRIM family, characterized by its conserved RING, B-box, and coiled-coil domains. It also contains a C-terminal PRY-SPRY domain, which enables its role as an intracellular antibody receptor. TRIM21 is widely expressed in mammalian cells and plays a critical role in innate immunity by targeting antibody-bound pathogens, such as viruses or bacteria, for proteasomal degradation via its E3 ubiquitin ligase activity. This process, termed "ubiquitin-dependent viral neutralization," links adaptive and innate immune responses by recognizing immune complexes that enter the cytosol during infection.
Recombinant TRIM21 proteins are engineered versions of the native protein, often produced in bacterial or mammalian expression systems for research and therapeutic applications. These proteins retain key functional domains, allowing scientists to study their interactions with immunoglobulins (IgG or IgM), ubiquitination mechanisms, and antiviral defense pathways in vitro. TRIM21's ability to bridge antibodies to the ubiquitin-proteasome system has also spurred interest in targeted protein degradation technologies, including PROTACs (Proteolysis-Targeting Chimeras), where it could serve as a modular component for directing disease-related proteins for destruction.
Beyond immunity, TRIM21 regulates cellular processes like autophagy, apoptosis, and inflammation, making its recombinant forms valuable tools for exploring these pathways. Dysregulation of TRIM21 has been implicated in autoimmune diseases (e.g., lupus) and cancer, highlighting its therapeutic potential. Researchers continue to optimize recombinant TRIM21 variants to enhance stability, specificity, and delivery for biomedical applications, positioning it as a versatile molecule at the intersection of immunology, molecular engineering, and drug development.
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