| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRBC1 |
| Uniprot No | P01850 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-129aa |
| 氨基酸序列 | DLNKVFPPEVAVFEPSEAEISHTQKATLVCLATGFFPDHVELSWWVNGKEVHSGVSTDPQPLKEQPALNDSRYCLSSRLRVSATFWQNPRNHFRCQVQFYGLSENDEWTQDRAKPVTQIVSAEAWGRAD |
| 预测分子量 | 19.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRBC1重组蛋白的参考文献示例(注：以下内容为模拟虚构，仅供参考格式，实际文献需通过学术数据库检索)：
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1. **文献名称**: *"Production and characterization of recombinant TRBC1 protein for T-cell receptor studies"*
**作者**: Zhang L, et al.
**摘要**: 本研究通过大肠杆菌表达系统成功制备了重组TRBC1蛋白，并通过质谱和Western blot验证其纯度与抗原性。该蛋白被用于分析T细胞受体(TCR)复合物的组装机制，揭示了TRBC1在稳定TCRβ链结构中的关键作用。
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2. **文献名称**: *"TRBC1-targeted antibodies generated by immunization with recombinant protein in murine models"*
**作者**: Smith JR, et al.
**摘要**: 利用重组TRBC1蛋白免疫小鼠，筛选出高特异性单克隆抗体。实验表明，这些抗体可特异性识别T细胞淋巴瘤细胞表面TRBC1.为肿瘤免疫治疗提供了潜在靶向工具。
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3. **文献名称**: *"Structural insights into TRBC1-mediated T-cell signaling via recombinant protein crystallography"*
**作者**: Tanaka K, et al.
**摘要**: 通过重组TRBC1蛋白的晶体结构解析，揭示了其与TCRα链的相互作用界面，阐明了TRBC1在T细胞活化信号转导中的分子机制，为设计TCR相关免疫调节药物提供了结构基础。
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4. **文献名称**: *"Recombinant TRBC1 as a diagnostic antigen for autoimmune T-cell disorders"*
**作者**: Gupta S, et al.
**摘要**: 研究评估了重组TRBC1蛋白在ELISA检测中的性能，发现其能高效检测患者血清中的抗TRBC1自身抗体，为自身免疫性T细胞疾病的诊断提供了新方法。
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**注意**：以上文献为示例性内容，实际研究中请通过PubMed、Web of Science等平台检索真实发表的论文。若需具体文献，建议结合关键词“TRBC1 recombinant protein”“T-cell receptor beta constant”进一步筛选。
TRBC1 (T Cell Receptor Beta Constant 1) is a critical component of the T cell receptor (TCR) complex, which plays a central role in adaptive immunity by recognizing antigenic peptides presented by major histocompatibility complex (MHC) molecules. As part of the β-chain of the TCR, TRBC1 contributes to the structural stability of the receptor and facilitates signal transduction upon antigen binding, enabling T cell activation and immune response coordination.
Recombinant TRBC1 protein is engineered in vitro using expression systems (e.g., bacterial, mammalian, or insect cells) to produce a purified, functional form of the native protein. This involves cloning the TRBC1 gene into expression vectors, optimizing production conditions, and employing purification techniques like affinity chromatography. The resulting protein often includes tags (e.g., His-tag) for easier isolation and detection.
In research, recombinant TRBC1 serves as a vital tool for studying TCR signaling mechanisms, T cell development, and immune dysregulation in diseases such as autoimmune disorders, lymphomas, and T cell-mediated cancers. It is also used to generate antibodies for diagnostic assays, including flow cytometry and immunohistochemistry, aiding in the identification of clonal T cell populations in malignancies. Recent therapeutic advancements explore TRBC1 as a target for engineered T cell therapies (e.g., CAR-T) or bispecific antibodies designed to selectively eliminate malignant T cells while sparing healthy ones. Its conserved sequence across TCR β-chains makes it a promising universal biomarker and therapeutic target in T cell-related pathologies. Ongoing studies continue to unravel its role in immune modulation and precision medicine applications.
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