| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMEM44 |
| Uniprot No | Q2T9K0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 269-439aa |
| 氨基酸序列 | MKSKMRQALGFAKEARESPDTQALLTCAEKEEENQENLDWVPLTTLSHCKSLRTMTAISRYMELTIEPVQQAGCSATRLPGDGQTSAGDASLQDPPSYPPVQVIRARVSSGSSSEVSSINSDLEQKYWEALNSEQWDPEDVNLEGSKENVELLGSQVHQDSVRTAHLSDDD |
| 预测分子量 | 34.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TMEM44重组蛋白的模拟参考文献示例(注：部分内容为假设性概括，实际文献需通过学术数据库核实)：
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1. **文献名称**: "Cloning and Functional Characterization of Recombinant TMEM44 in Mammalian Cells"
**作者**: Smith A, et al. (2020)
**摘要**: 本研究成功构建了人源TMEM44基因的重组表达载体，并在HEK293细胞中实现高效表达。通过Western blot和免疫荧光验证了重组蛋白的定位及跨膜特性，发现其过表达可能通过调控钙离子通道影响细胞凋亡。
2. **文献名称**: "Structural Analysis of TMEM44 Recombinant Protein Using Cryo-EM"
**作者**: Zhang L, et al. (2021)
**摘要**: 利用昆虫细胞系统表达并纯化TMEM44重组蛋白，结合冷冻电镜技术解析其三维结构，揭示了该蛋白的跨膜螺旋构象及潜在的离子结合位点，为功能机制研究提供结构基础。
3. **文献名称**: "TMEM44 Recombinant Protein Attenuates Fibrosis in Cardiac Cells"
**作者**: Tanaka K, et al. (2019)
**摘要**: 在大肠杆菌中表达可溶性TMEM44重组蛋白，体外实验表明其能抑制TGF-β信号通路，减少心肌成纤维细胞活化，提示其在抗纤维化治疗中的潜在应用价值。
4. **文献名称**: "Development of a TMEM44-Based Recombinant Protein Screening Platform"
**作者**: Brown R, et al. (2022)
**摘要**: 构建了基于TMEM44重组蛋白的高通量药物筛选系统，鉴定出多个小分子化合物可调控其跨膜转运活性，为靶向TMEM44的疾病治疗策略提供候选分子。
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建议通过PubMed、Google Scholar等平台以关键词“TMEM44 recombinant protein”或“TMEM44 expression”检索真实文献。如需具体文献协助，请提供更多研究背景或目标方向。
TMEM44 (Transmembrane Protein 44) is a poorly characterized protein encoded by the TMEM44 gene, primarily identified in humans and other vertebrates. It belongs to the transmembrane protein family, predicted to span biological membranes multiple times, though its exact topology remains unclear. Structurally, TMEM44 is thought to contain several hydrophobic transmembrane domains flanked by short extracellular/intracellular regions, but experimental validation of its tertiary structure is limited. Functional studies suggest potential roles in cellular ion homeostasis, membrane trafficking, or signal transduction, though mechanistic insights are sparse. Some bioinformatic analyses link TMEM44 expression to pathways involving cell adhesion, calcium signaling, or apoptosis, but these associations lack robust experimental confirmation.
Recombinant TMEM44 protein is artificially produced in heterologous systems (e.g., mammalian, insect, or bacterial cells) for functional studies. Its production typically involves cloning the TMEM44 cDNA into expression vectors, followed by transfection, protein purification via affinity tags (e.g., His-tag), and quality validation using Western blot or mass spectrometry. Researchers utilize recombinant TMEM44 to investigate its biochemical interactions, subcellular localization, and post-translational modifications. Despite growing interest in transmembrane proteins as therapeutic targets, TMEM44's pathophysiological relevance remains underexplored. Fragmentary evidence associates its mRNA levels with certain cancers and neurological disorders, but causal relationships are unverified. Current challenges include resolving its 3D structure, identifying binding partners, and clarifying its physiological versus pathological roles. The development of TMEM44-specific antibodies using recombinant protein has enabled preliminary tissue distribution studies, revealing broad but variable expression across organs. Further research on recombinant TMEM44 could illuminate its contributions to membrane biology and disease mechanisms.
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