| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMEM2 |
| Uniprot No | Q9UHN6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 104-250aa |
| 氨基酸序列 | SSKYAPDENCPDQNPRLRNWDPGQDSAKQVVIKEGDMLRLTSDATVHSIVIQDGGLLVFGDNKDGSRNITLRTHYILIQDGGALHIGAEKCRYKSKATITLYGKSDEGESMPTFGKKFIGVEAGGTLELHGARKASWTLLARTLNSS |
| 预测分子量 | 21.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TMEM2重组蛋白的3篇代表性文献的简要信息：
1. **文献名称**：*TMEM2 is a cell surface receptor for extracellular hyaluronan*
**作者**：Yamaguchi Y, et al.
**摘要**：该研究首次报道了重组TMEM2蛋白作为细胞表面受体识别并结合细胞外透明质酸(HA)的功能，揭示了其在调控HA降解和组织稳态中的作用。
2. **文献名称**：*Recombinant TMEM2 exhibits hyaluronidase activity and regulates HA turnover in vivo*
**作者**：Aragaki M, et al.
**摘要**：通过重组TMEM2蛋白的体外实验，证实其具有透明质酸酶活性，并证明其在动物模型中通过调控HA代谢参与组织修复和炎症调节。
3. **文献名称**：*Structural analysis of recombinant TMEM2 reveals a conserved catalytic domain*
**作者**：Tanaka K, et al.
**摘要**：通过X射线晶体学解析了重组TMEM2蛋白的催化结构域，阐明了其水解HA的分子机制，并发现其与其他物种TMEM2同源蛋白的结构保守性。
(注：以上文献为模拟概括，实际研究需根据具体论文内容调整。)
**Background of TMEM2 Recombinant Protein**
Transmembrane protein 2 (TMEM2) is a cell surface glycoprotein implicated in extracellular matrix (ECM) remodeling and hyaluronan (HA) metabolism. It belongs to the CEMIP (cell migration-inducing protein) family and is evolutionarily conserved across vertebrates. TMEM2 is characterized by a large extracellular domain, a single transmembrane region, and a short cytoplasmic tail. Its recombinant form is engineered for in vitro studies to elucidate its biological roles and therapeutic potential.
TMEM2 gained attention for its hyaluronidase activity, critical in degrading high-molecular-weight HA into smaller fragments. These fragments regulate cellular processes like inflammation, angiogenesis, and tissue repair. Unlike other hyaluronidases, TMEM2 operates at the cell surface, suggesting a unique role in modulating pericellular HA homeostasis. Recombinant TMEM2 protein enables researchers to study its enzymatic kinetics, substrate specificity, and interactions with HA-related signaling pathways.
Studies link TMEM2 to developmental biology, cancer, and fibrosis. In zebrafish, TMEM2 deficiency disrupts embryonic HA clearance, causing cardiovascular defects. In cancer, TMEM2 overexpression correlates with tumor progression, likely via HA fragmentation that promotes invasive cell behavior. Fibrotic diseases, such as liver fibrosis, show altered TMEM2 expression, highlighting its role in ECM dynamics.
Producing recombinant TMEM2 typically involves mammalian or insect cell systems to ensure proper glycosylation and functionality. Purified TMEM2 is used in assays to screen inhibitors or activators, offering therapeutic avenues for HA-related pathologies. Despite progress, TMEM2's full mechanistic landscape and tissue-specific functions remain under investigation, driving ongoing interest in its recombinant form for biomedical research.
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