Recombinant Human TMEM141 protein

**Background of TMEM141 Recombinant Protein**

TMEM141 (Transmembrane Protein 141) is a poorly characterized protein encoded by the *TMEM141* gene, which is conserved across vertebrates and located on human chromosome 4q25. It belongs to the TMEM family, a group of membrane-spanning proteins with diverse cellular functions, though many remain understudied. TMEM141 is predicted to contain multiple transmembrane domains, suggesting its role as an integral membrane protein, potentially localized to mitochondria or other organelles. Limited studies indicate its involvement in cellular stress responses, mitochondrial function, and apoptosis regulation.

Recombinant TMEM141 protein is engineered to enable functional and structural studies. It is typically produced using heterologous expression systems (e.g., *E. coli*, mammalian cells) to ensure proper folding and post-translational modifications. The recombinant form allows researchers to investigate TMEM141's biochemical properties, interactome, and role in pathways such as oxidative stress or mitochondrial dynamics. Recent interest in TMEM141 stems from its potential link to neurological disorders and cancer. For instance, altered expression levels have been observed in glioblastoma and hepatocellular carcinoma, hinting at a possible role in tumor progression or therapy resistance.

Despite its emerging relevance, TMEM141's precise molecular mechanisms remain elusive. Recombinant protein tools are critical for unraveling its physiological and pathological functions, including its interaction with mitochondrial membranes, ion channels, or apoptotic regulators. Such research may inform therapeutic strategies targeting TMEM141-associated pathways in disease contexts. Current challenges include validating its subcellular localization, resolving its 3D structure, and identifying binding partners—areas where recombinant protein-based assays are indispensable.

In summary, TMEM141 recombinant protein serves as a vital resource for decoding the enigmatic roles of this transmembrane protein in health and disease.