| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TIMM23 |
| Uniprot No | O14925 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-209aa |
| 氨基酸序列 | MEGGGGSGNKTTGGLAGFFGAGGAGYSHADLAGVPLTGMNPLSPYLNVDPRYLVQDTDEFILPTGANKTRGRFELAFFTIGGCCMTGAAFGAMNGLRLGLKETQNMAWSKPRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRGAEDDLNTVAAGTMTGMLYKCTGGLRGIARGGLTGLTLTSLYALYNNWEHMKGSLLQQSL |
| 预测分子量 | 48.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TIMM23重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*The Tim23 core complex functions as a mitochondrial voltage-gated translocase*
**作者**：Bauer, M.F., Sirrenberg, C., Neupert, W., & Brunner, M.
**摘要**：该研究通过重组表达TIMM23及其亚基TIMM17.证明其形成的核心复合体依赖线粒体膜电位，调控前体蛋白向线粒体内膜的转运，揭示了其作为电压依赖性通道的功能机制。
2. **文献名称**：*Tim23 links the inner and outer mitochondrial membranes*
**作者**：Dekker, P.J., Ryan, M.T., Brix, J., Müller, H., Hönlinger, A., & Pfanner, N.
**摘要**：利用重组TIMM23蛋白实验，发现其与TIMM17共纯化形成复合物，并直接与外膜转运复合体TOM相互作用，阐明了TIMM23在跨线粒体膜蛋白转运中的桥接作用。
3. **文献名称**：*Dynamic assembly of the mitochondrial protein import channel*
**作者**：Mokranjac, D., Bourenkov, G., Hell, K., Neupert, W., & Groll, M.
**摘要**：通过重组TIMM23与TIMM50的共表达，揭示了其在膜电位驱动下构象变化的分子机制，证实重组蛋白复合体对前体蛋白的识别与转运的调控作用。
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以上文献均聚焦于TIMM23重组蛋白的功能、复合体组装及其在线粒体蛋白转运中的核心作用，涉及结构、动态机制及跨膜协作。
The TIMM23 (Translocase of the Inner Mitochondrial Membrane 23) recombinant protein is a key component of the TIM23 complex, which plays a critical role in mitochondrial protein import. Mitochondria rely on specialized translocases to import nuclear-encoded proteins, as the organelle’s genome encodes only a small fraction of its proteome. The TIM23 complex, located in the inner mitochondrial membrane, mediates the translocation of presequence-containing precursor proteins from the mitochondrial intermembrane space into the mitochondrial matrix. TIMM23 serves as the central pore-forming subunit, interacting with other subunits like TIMM17 and TIMM50 to facilitate precursor recognition, membrane potential-dependent translocation, and cooperation with matrix Hsp70 for protein folding.
Recombinant TIMM23 is engineered to study the molecular mechanisms of mitochondrial protein import in vitro. Researchers produce it using heterologous expression systems (e.g., *E. coli* or mammalian cell cultures) to obtain purified, functional protein for structural and functional analyses. Its recombinant form allows detailed investigation of its role in substrate recognition, channel gating, and energy-dependent transport. Studies using TIMM23 recombinant proteins have elucidated how the TIM23 complex distinguishes between precursor proteins, coordinates with the TIM44–mtHsp70 motor system, and responds to membrane potential changes.
Dysregulation of TIMM23 is linked to mitochondrial dysfunction, implicated in neurodegenerative diseases, cancer, and metabolic disorders. Recombinant TIMM23 enables drug discovery efforts targeting mitochondrial protein import pathways. Additionally, structural studies (e.g., cryo-EM) using recombinant TIMM23 have revealed conformational changes during substrate translocation, providing insights into the dynamic regulation of mitochondrial biogenesis. Overall, TIMM23 recombinant proteins serve as indispensable tools for dissecting mitochondrial import machinery and developing therapeutic strategies for mitochondrial diseases.
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