| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TIMM17B |
| Uniprot No | O60830 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-172aa |
| 氨基酸序列 | MEEYAREPCPWRIVDDCGGAFTMGVIGGGVFQAIKGFRNAPVGIRHRLRGSANAVRIRAPQIGGSFAVWGGLFSTIDCGLVRLRGKEDPWNSITSGALTGAVLAARSGPLAMVGSAMMGGILLALIEGVGILLTRYTAQQFRNAPPFLEDPSQLPPKDGTPAPGYPSYQQYH |
| 预测分子量 | 45.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TIMM17B重组蛋白的3篇文献示例(内容为虚构模拟，供参考)：
1. **文献名称**：*Functional characterization of recombinant TIMM17B in mitochondrial protein import*
**作者**：Schneider A., et al.
**摘要**：研究利用大肠杆菌表达系统重组表达TIMM17B蛋白，验证其在线粒体内膜转位酶复合体(TIM23复合体)中的作用，证明其与TIMM23的相互作用对前体蛋白的跨膜转运至关重要。
2. **文献名称**：*TIMM17B overexpression alters mitochondrial membrane dynamics in cancer cells*
**作者**：Chen L., et al.
**摘要**：通过哺乳动物细胞重组表达TIMM17B，发现其过表达导致线粒体膜电势异常，并促进癌细胞凋亡，提示其在肿瘤治疗中的潜在应用价值。
3. **文献名称**：*Structural insights into TIMM17B mutations linked to neurodegenerative disorders*
**作者**：Yamamoto K., et al.
**摘要**：利用重组TIMM17B蛋白解析了两种致病突变体(R122H和G89D)的晶体结构，揭示突变破坏其与线粒体前体蛋白的结合能力，可能与帕金森病相关。
注：以上文献为示例，实际研究中请通过PubMed或Google Scholar以“TIMM17B recombinant protein”等关键词检索最新文献。
**Background of TIMM17B Recombinant Protein**
TIMM17B (Translocase of Inner Mitochondrial Membrane 17 Homolog B) is a key component of the TIM23 complex, a critical protein transport machinery embedded in the mitochondrial inner membrane. This complex facilitates the import of nuclear-encoded precursor proteins—particularly those with N-terminal presequences—into the mitochondrial matrix, a process essential for maintaining mitochondrial function, energy production, and cellular homeostasis. TIMM17B, along with its paralog TIMM17A, forms the core channel of the TIM23 complex, mediating precursor protein recognition and membrane translocation.
Structurally, TIMM17B contains four transmembrane domains and interacts with other TIM23 subunits, such as TIMM23 and TIMM44. to coordinate ATP-dependent protein translocation. Its role is particularly vital in stress-responsive tissues, as TIMM17B expression is upregulated under conditions like hypoxia or metabolic stress, suggesting its involvement in adaptive mitochondrial responses. Dysregulation of TIMM17B has been linked to mitochondrial disorders, neurodegenerative diseases, and cancer, highlighting its biomedical relevance.
Recombinant TIMM17B protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cell lines) to produce purified, functional protein for *in vitro* studies. This recombinant tool enables researchers to dissect TIMM17B's molecular interactions, structural dynamics, and role in mitochondrial import mechanisms. It also aids in screening therapeutic compounds targeting mitochondrial dysfunction or studying disease-associated mutations. By providing a controlled, high-purity protein source, recombinant TIMM17B accelerates mechanistic studies and translational applications in mitochondrial biology and pathology.
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