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Recombinant Human TMEM14B protein

  • 中文名: 跨膜蛋白14B(TMEM14B)重组蛋白
  • 别    名: TMEM14B;Transmembrane protein 14B
货号: PA2000-2254
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点TMEM14B
Uniprot No Q9NUH8
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间 1-114aa
氨基酸序列MEKPLFPLVPLHWFGFGYTALVVSGGIVGYVKTGSVPSLAAGLLFGSLAGLGAYQLYQDPRNVWGFLAATSVTFVGVMGMRSYYYGKFMPVGLIAGASLLMAAKVGVRMLMTSD
预测分子量 39.1 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于TMEM14B重组蛋白的虚构参考文献示例(注:实际文献需通过学术数据库检索获取):

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1. **文献名称**:*Expression and Functional Analysis of Recombinant TMEM14B in Mitochondrial Apoptosis Regulation*

**作者**:Zhang X, et al.

**摘要**:本研究通过原核表达系统成功制备了TMEM14B重组蛋白,并验证其在线粒体膜上的定位。实验表明,该蛋白通过抑制细胞色素C释放调控凋亡,为癌症治疗提供了新靶点。

2. **文献名称**:*Structural Characterization of Recombinant TMEM14B and Its Role in Ion Transport*

**作者**:Smith J, et al.

**摘要**:利用冷冻电镜解析了TMEM14B重组蛋白的跨膜结构,揭示其潜在的离子通道功能。实验显示该蛋白参与细胞内钙离子稳态调节,可能影响神经退行性疾病进程。

3. **文献名称**:*TMEM14B Overexpression in Hepatocellular Carcinoma and Recombinant Protein Screening*

**作者**:Li Y, et al.

**摘要**:在肝癌细胞中高表达TMEM14B重组蛋白,发现其通过激活AKT信号通路促进细胞增殖。研究为肝癌的分子机制和靶向治疗提供了依据。

4. **文献名称**:*Interaction of Recombinant TMEM14B with Autophagy-Related Proteins*

**作者**:Chen L, et al.

**摘要**:通过重组蛋白互作实验,证实TMEM14B与自噬蛋白Beclin-1结合,调控细胞自噬过程,可能影响肿瘤耐药性。

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**建议**:以上内容为示例,实际研究中请通过**PubMed**、**Google Scholar**或**Web of Science**检索关键词“TMEM14B recombinant protein”获取真实文献。如需进一步协助,可提供具体研究方向(如结构、功能或疾病关联),我将帮助优化检索策略。

背景信息

TMEM14B (Transmembrane Protein 14B) is a conserved eukaryotic protein belonging to the TMEM14 family, characterized by its predicted transmembrane domains and mitochondrial localization. It is primarily localized to the inner mitochondrial membrane and has been implicated in regulating mitochondrial function, particularly in processes linked to apoptosis and cellular energy metabolism. While its exact molecular mechanisms remain under investigation, studies suggest TMEM14B interacts with components of the mitochondrial permeability transition pore (MPTP), influencing cytochrome c release and caspase activation during programmed cell death. Dysregulation of TMEM14B expression has been observed in certain cancers and neurodegenerative disorders, highlighting its potential role in disease pathogenesis.

Recombinant TMEM14B protein is engineered for in vitro studies to elucidate its structure-function relationships and therapeutic potential. Typically produced in Escherichia coli or mammalian expression systems, the recombinant protein retains key functional domains, enabling investigations into its interaction partners, post-translational modifications, and role in mitochondrial dynamics. Purification often involves affinity tags (e.g., His-tag) followed by chromatography techniques. Researchers utilize this tool in apoptosis assays, mitochondrial membrane potential measurements, and drug screening platforms targeting apoptosis-related pathways. Recent studies also explore its involvement in metabolic reprogramming and ferroptosis, expanding its relevance to broader cellular stress responses. Despite progress, further work is needed to map its tissue-specific isoforms and validate its candidacy as a diagnostic biomarker or therapeutic target.

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