Recombinant Human TMEM14B protein

TMEM14B (Transmembrane Protein 14B) is a conserved eukaryotic protein belonging to the TMEM14 family, characterized by its predicted transmembrane domains and mitochondrial localization. It is primarily localized to the inner mitochondrial membrane and has been implicated in regulating mitochondrial function, particularly in processes linked to apoptosis and cellular energy metabolism. While its exact molecular mechanisms remain under investigation, studies suggest TMEM14B interacts with components of the mitochondrial permeability transition pore (MPTP), influencing cytochrome c release and caspase activation during programmed cell death. Dysregulation of TMEM14B expression has been observed in certain cancers and neurodegenerative disorders, highlighting its potential role in disease pathogenesis.

Recombinant TMEM14B protein is engineered for in vitro studies to elucidate its structure-function relationships and therapeutic potential. Typically produced in Escherichia coli or mammalian expression systems, the recombinant protein retains key functional domains, enabling investigations into its interaction partners, post-translational modifications, and role in mitochondrial dynamics. Purification often involves affinity tags (e.g., His-tag) followed by chromatography techniques. Researchers utilize this tool in apoptosis assays, mitochondrial membrane potential measurements, and drug screening platforms targeting apoptosis-related pathways. Recent studies also explore its involvement in metabolic reprogramming and ferroptosis, expanding its relevance to broader cellular stress responses. Despite progress, further work is needed to map its tissue-specific isoforms and validate its candidacy as a diagnostic biomarker or therapeutic target.