| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | mppA |
| Uniprot No | P77348 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-537aa |
| 氨基酸序列 | AEVPSGTVLAEKQELVRHIKDEPASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNAKWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTAVDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERVVNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKLLKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPAWHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQKIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLLTSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNGRLIKPWLKGYPINNPEDVAYSRTMYIVKH |
| 预测分子量 | 73.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与mppA重组蛋白相关的文献摘要概括(注：文献为示例性构建，非真实存在)：
1. **《Cloning and expression of mppA gene from Escherichia coli》**
- 作者：Zhang Y, et al.
- 摘要：报道了大肠杆菌mppA基因的克隆及在大肠杆菌BL21中的重组表达，优化了IPTG诱导条件并通过Ni柱纯化获得可溶性蛋白，验证了其结合小肽的生物学活性。
2. **《Structural analysis of recombinant MppA protein by X-ray crystallography》**
- 作者：Smith J, et al.
- 摘要：解析了重组mppA蛋白的晶体结构，揭示了其周质结合蛋白典型的三维构象及配体结合口袋的关键氨基酸残基，为理解其转运机制提供结构基础。
3. **《Functional characterization of MppA in bacterial peptide uptake》**
- 作者：Lee H, et al.
- 摘要：通过构建mppA缺失株及回补重组蛋白实验，证实mppA在寡肽摄取中的核心作用，并证明其与内膜转运蛋白MppB/C的协同功能。
4. **《High-yield production of recombinant MppA using fed-batch fermentation》**
- 作者：Wang L, et al.
- 摘要：开发了基于毕赤酵母系统的mppA重组蛋白高密度发酵工艺，通过补料策略使蛋白产量提高至1.2 g/L，为工业级应用奠定基础。
(注：以上内容为模拟文献摘要，实际研究中建议通过PubMed/Google Scholar以"mppA recombinant protein"等关键词检索真实文献。)
**Background of mppA Recombinant Protein**
The mppA gene encodes a periplasmic substrate-binding protein associated with the MppA-OppBCDF system, a bacterial oligopeptide transporter critical for nutrient uptake and environmental adaptation. Originally identified in *Escherichia coli*, MppA binds small peptides and murein tripeptides, playing roles in peptidoglycan recycling, osmotic stress response, and nutrient scavenging. Its function is linked to maintaining cell envelope integrity and facilitating survival under nutrient-limited conditions.
Recombinant mppA protein is produced via heterologous expression systems (e.g., *E. coli* or yeast), often fused with tags like His-tag for purification. This engineered protein retains its native ligand-binding properties, making it valuable for structural and functional studies. Researchers utilize mppA recombinant protein to investigate bacterial peptide transport mechanisms, host-pathogen interactions, and stress response pathways. Its crystal structure has been resolved, revealing a conserved bilobed architecture with a central ligand-binding pocket, aiding in understanding substrate specificity and conformational changes during transport.
Beyond basic research, mppA recombinant protein has applications in antimicrobial drug development. Targeting peptide transport systems like MppA-Opp could disrupt bacterial survival strategies, offering novel therapeutic avenues. It also serves as a model for studying ABC transporters in higher organisms. Additionally, engineered mppA variants are explored in biotechnology for biosensors or synthetic biology tools. Overall, mppA recombinant protein bridges fundamental microbiology and applied research, enhancing insights into bacterial physiology and potential clinical interventions.
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