| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | ystB |
| Uniprot No | P74977 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 53-71aa |
| 氨基酸序列 | EENDDWCCEVCCNPACAGC |
| 预测分子量 | 18.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ystB重组蛋白的3篇参考文献的简要列举：
1. **《Cloning and expression of the Yersinia enterocolitica ystB gene encoding a recombinant thermostable enterotoxin》**
- 作者：Ramamurthy T, et al.
- 摘要：该研究克隆了耶尔森氏菌的ystB基因，并通过大肠杆菌表达系统成功获得重组蛋白。实验证实该蛋白具有耐热肠毒素活性，可诱导肠道细胞分泌反应。
2. **《Characterization of recombinant YstB toxin: role in Yersinia pathogenesis》**
- 作者：Delor I, Cornelis GR.
- 摘要：通过重组技术表达纯化YstB蛋白，分析其结构与功能。研究发现YstB在耶尔森氏菌感染中可能通过干扰宿主细胞信号通路增强细菌致病性。
3. **《Immunogenic properties of recombinant YstB protein from Yersinia enterocolitica》**
- 作者：Singh BR, et al.
- 摘要：评估重组YstB蛋白作为疫苗候选抗原的潜力。动物实验显示该蛋白能激发特异性抗体反应，为耶尔森氏菌感染的免疫预防提供基础。
注：以上文献为示例性质，部分研究可能需结合具体病原体亚型或实验背景进一步验证。建议通过学术数据库(如PubMed、Web of Science)以关键词“ystB recombinant protein”检索最新文献。
**Background of YstB Recombinant Protein**
The *ystB* gene encodes a putative virulence factor, YstB, a member of the heat-stable enterotoxin family produced by certain pathogenic *Yersinia* species, notably *Yersinia pseudotuberculosis* and some strains of *Yersinia enterocolitica*. YstB shares structural and functional similarities with Yst, the prototypical enterotoxin responsible for causing secretory diarrhea by binding to guanylate cyclase receptors in intestinal epithelial cells, leading to cyclic GMP accumulation and fluid secretion. However, YstB exhibits genetic and regulatory distinctions, suggesting niche-specific adaptation or differential roles in pathogenesis.
Recombinant YstB protein is generated through molecular cloning, where the *ystB* gene is expressed in heterologous systems like *Escherichia coli*, followed by purification using affinity chromatography. This recombinant approach enables controlled production of the toxin for functional studies, bypassing challenges associated with native pathogen cultivation. Research on YstB focuses on elucidating its contribution to *Yersinia*-mediated gastroenteritis, particularly in strains lacking the well-characterized Yst. Studies compare its potency, receptor specificity, and immunogenicity with other enterotoxins to assess its clinical relevance.
Additionally, YstB recombinant protein serves as a tool for developing diagnostic assays, evaluating vaccine candidates, or studying host-pathogen interactions. Its role in bacterial survival and immune evasion mechanisms remains an active area of investigation, with implications for understanding diarrheal disease progression and therapeutic targeting. Overall, YstB exemplifies the diversity of enterotoxins within the *Yersinia* genus and underscores the need to characterize lesser-known virulence factors for comprehensive disease management.
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