| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | alaC |
| Uniprot No | P77434 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-412aa |
| 氨基酸序列 | MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMGNPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAIVTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNELERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVYDGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYGTFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKIPEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKAMFRADGLLPASSKHIHENAE |
| 预测分子量 | 62.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于alaC重组蛋白的模拟参考文献示例(注：以下内容为假设性示例，非真实文献)：
---
1. **《Heterologous Expression and Characterization of alaC-Encoded Alanine Racemase from Bacillus subtilis》**
- Authors: Smith J, et al.
- 摘要：研究报道了枯草芽孢杆菌alaC基因编码的丙氨酸消旋酶在大肠杆菌中的异源表达，优化了纯化条件并分析了其酶学性质，证实其在D-丙氨酸生物合成中的关键作用。
2. **《Crystal Structure of Recombinant alaC Protein Reveals Substrate Binding Mechanism》**
- Authors: Lee H, Kim S.
- 摘要：通过X射线晶体学解析了重组alaC蛋白的三维结构，揭示了其与底物L-丙氨酸的结合位点及催化机制，为设计靶向抑制剂提供了结构基础。
3. **《Functional Role of alaC in Bacterial Cell Wall Biosynthesis: Insights from Recombinant Protein Knockout Studies》**
- Authors: Zhang Y, et al.
- 摘要：利用alaC重组蛋白敲除模型，证明该蛋白在细菌细胞壁肽聚糖合成中不可或缺，缺失导致细菌生长抑制，提示其作为潜在抗菌靶点。
4. **《Biotechnological Application of alaC Recombinant Protein in Enzymatic Synthesis of D-Amino Acids》**
- Authors: Johnson R, et al.
- 摘要：开发了基于alaC重组蛋白的固定化酶系统，用于高效催化L-丙氨酸转化为D-丙氨酸，展示了其在工业级D-氨基酸生产中的应用潜力。
---
如需真实文献，建议通过PubMed或Web of Science以“alaC recombinant protein”“alanine racemase expression”等关键词检索，并核实具体研究内容。
**Background of AlaC Recombinant Protein**
The AlaC recombinant protein is a genetically engineered biomolecule derived from the *alaC* gene, which encodes a key enzyme involved in microbial metabolic pathways, particularly in the biosynthesis of alanine or related amino acids. Originally identified in bacteria such as *Escherichia coli* or other prokaryotes, the *alaC* gene product plays a role in amino acid metabolism, potentially contributing to stress adaptation, nitrogen assimilation, or energy production. Recombinant AlaC is produced via heterologous expression systems, such as *E. coli* or yeast, enabling large-scale purification for research or industrial applications.
Interest in AlaC stems from its enzymatic properties, which may be leveraged in biotechnological processes, including the production of chiral compounds, biofuels, or pharmaceutical precursors. Its ability to catalyze specific reactions under mild conditions aligns with sustainable chemistry goals. Structural studies of AlaC have provided insights into its active sites and substrate-binding mechanisms, facilitating protein engineering to enhance stability, activity, or substrate specificity.
AlaC recombinant protein also serves as a model for studying microbial metabolism and enzyme evolution. Its role in cellular resilience under nutrient-limited or high-stress environments has implications for understanding microbial survival strategies. Additionally, AlaC-based assays are utilized in metabolic pathway analysis or drug discovery targeting pathogenic microbes. Ongoing research explores its potential in synthetic biology frameworks or as a biocatalyst in industrial enzymology, highlighting its versatility across disciplines.
×
