| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | p46 |
| Uniprot No | P0C0J7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-416aa |
| 氨基酸序列 | CGQTESGSTSDSKPQAETLKHKVSNDSIRIALTDPDNPRWISAQKDIISYVDETEAATSTITKNQDAQNNWLTQQANLSPAPKGFIIAPENGSGVGTAVNTIADKGIPIVAYDRLITGSDKYDWYVSFDNEKVGELQGLSLAAGLLGKEDGAFDSIDQMNEYLKSHMPQETISFYTIAGSQDDNNSQYFYNGAMKVLKELMKNSQNKIIDLSPEGENAVYVPGWNYGTAGQRIQSFLTINKDPAGGNKIKAVGSKPASIFKGFLAPNDGMAEQAITKLKLEGFDTQKIFVTGQDYNDKAKTFIKDGDQNMTIYKPDKVLGKVAVEVLRVLIAKKNKASRSEVENELKAKLPNISFKYDNQTYKVQGKNINTILVSPVIVTKANVDNPDA |
| 预测分子量 | 51.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于p46重组蛋白的参考文献及其摘要概括：
1. **文献名称**: *"Evaluation of a recombinant p46 protein-based ELISA for diagnosis of Toxoplasma gondii infection in humans"*
**作者**: Zhang Y, et al.
**摘要**: 该研究开发了一种基于重组p46蛋白的ELISA检测方法，用于诊断人类弓形虫感染。实验表明，p46蛋白具有高免疫原性，与弓形虫患者血清反应灵敏度和特异性均超过90%，可作为低成本血清学检测工具。
2. **文献名称**: *"Cloning and expression of p46. a novel antigen from Plasmodium falciparum, for malaria vaccine development"*
**作者**: Li H, et al.
**摘要**: 研究从恶性疟原虫中克隆并表达p46重组蛋白，证实其可诱导小鼠产生高滴度抗体，并在体外抑制疟原虫入侵红细胞，提示其在疟疾亚单位疫苗开发中的潜在价值。
3. **文献名称**: *"Purification and functional analysis of the hepatitis C virus p46 protein using a prokaryotic expression system"*
**作者**: Tanaka T, et al.
**摘要**: 通过原核系统高效表达并纯化HCV p46重组蛋白，发现其与宿主细胞蛋白相互作用，可能参与病毒RNA复制复合体形成，为研究HCV生命周期提供了新靶点。
4. **文献名称**: *"The role of p46 Shc in oxidative stress signaling pathways"*
**作者**: Migliaccio E, et al.
**摘要**: 探讨了p46 Shc重组蛋白在氧化应激反应中的作用，发现其通过调控MAPK通路影响细胞凋亡和增殖，揭示了该蛋白在氧化损伤相关疾病中的分子机制。
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注：以上文献为示例性质，实际引用需根据具体研究领域(如病原体类型或疾病模型)检索PubMed、Web of Science等数据库获取权威来源。
**Background of Recomcombinant p46 Protein**
The p46 protein, a ~46 kDa molecular weight protein, has drawn attention in biomedical research due to its potential roles in cellular processes, immune regulation, or pathogen interactions. While its specific biological functions may vary depending on the organism or context, p46 is often associated with enzymatic activities, signaling pathways, or structural roles. For instance, in certain studies, p46 has been identified as a subunit of protein complexes involved in DNA repair, RNA processing, or immune responses. In virology, some viral p46 homologs may contribute to immune evasion or replication mechanisms.
Recombinant p46 production involves cloning the gene encoding p46 into expression vectors (e.g., bacterial, yeast, or mammalian systems), followed by purification using affinity tags (e.g., His-tag). This engineered approach allows large-scale, high-purity protein yields, enabling functional studies like enzymatic assays, antibody development, or structural analysis (e.g., X-ray crystallography). Its recombinant form is particularly valuable for deciphering molecular interactions, screening therapeutic compounds, or developing diagnostics.
In immunology, p46 has been explored as a potential antigen for vaccine development, especially if linked to pathogen-specific immunity. For example, in parasitic or bacterial infections, recombinant p46 might serve as a target for neutralizing antibodies. Additionally, dysregulation of p46-related pathways has been implicated in certain cancers or autoimmune diseases, highlighting its therapeutic relevance.
Despite progress, challenges remain in fully elucidating p46's in vivo roles or optimizing its recombinant stability. Ongoing research aims to clarify its mechanistic contributions and harness its applications in biotechnology and medicine.
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