| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | iagB |
| Uniprot No | P0CL15 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-160aa |
| 氨基酸序列 | DCWLQAEKMFNIESELLYAIAQQESAMKPGAIGHNRDGSTDLGLMQINSFHMKRLKKMGISEKQLLQDPCISVIVGASILSDMMKIYGYSWEAVGAYNAGTSPKRSDIRKRYAKKIWENYRKLKGMSAEEKNKRLSIAANK |
| 预测分子量 | 32.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于iagB重组蛋白的假设性参考文献示例(请注意，这些信息为模拟生成，实际文献需通过学术数据库确认)：
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1. **文献名称**: *Cloning and Immunogenicity Assessment of Recombinant iagB Protein from Vibrio parahaemolyticus*
**作者**: Zhang Y, et al.
**摘要**: 本研究成功克隆并表达了副溶血性弧菌的iagB基因，纯化的重组蛋白在小鼠模型中诱导了显著的抗体反应，表明其作为疫苗候选的潜力。
2. **文献名称**: *Functional Characterization of iagB in Biofilm Formation of Klebsiella pneumoniae*
**作者**: Lee S, et al.
**摘要**: 通过重组iagB蛋白的功能分析，揭示了该蛋白在肺炎克雷伯菌生物膜形成中的关键作用，为抗感染策略提供了新靶点。
3. **文献名称**: *Diagnostic Potential of iagB Recombinant Antigen in Salmonella Detection*
**作者**: Smith J, et al.
**摘要**: 研究评估了iagB重组蛋白作为沙门氏菌血清学诊断抗原的效能，显示其高特异性，适用于快速检测方法的开发。
4. **文献名称**: *Expression Optimization and Purification of iagB Recombinant Protein in E. coli*
**作者**: Wang H, et al.
**摘要**: 报道了一种高效表达和纯化iagB重组蛋白的大肠杆菌系统，为后续结构研究和应用奠定了基础。
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**建议**：如需真实文献，请通过PubMed、Google Scholar等平台搜索关键词“iagB recombinant protein”或联系相关领域数据库获取最新研究。
**Background of IagB Recombinant Protein**
The IagB protein is a virulence-associated factor initially identified in pathogenic bacteria such as *Salmonella enterica* serovar Typhimurium. It is encoded by the *iagB* gene, located within the *Salmonella* pathogenicity island 1 (SPI-1), a genomic region critical for bacterial invasion into host cells. SPI-1 encodes components of the type III secretion system (T3SS), which injects effector proteins into host cells to manipulate cellular processes, facilitating bacterial entry and survival. IagB is implicated in modulating host cell membrane dynamics, potentially contributing to the formation of invasion-related structures or membrane ruffling during infection.
Recombinant IagB protein is produced through genetic engineering, typically by cloning the *iagB* gene into expression vectors (e.g., pET or pGEX systems) and expressing it in bacterial hosts like *E. coli* BL21(DE3). Purification methods such as affinity chromatography (e.g., His-tag systems) ensure high yield and purity. Studies on recombinant IagB aim to dissect its structural and functional roles in pathogenesis, including interactions with host proteins or lipids.
Research highlights IagB's potential involvement in phosphatidylglycerol synthase activity, suggesting a role in bacterial membrane lipid synthesis or host membrane modification. Its recombinant form is also explored for vaccine development, as it may elicit protective immune responses. Additionally, IagB serves as a tool for studying SPI-1-mediated virulence mechanisms and screening antimicrobial agents targeting T3SS components. Despite progress, its precise molecular mechanisms and therapeutic applications require further investigation.
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