| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | ACT1 |
| Uniprot No | P60010 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-375aa |
| 氨基酸序列 | MDSEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQSKRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMTQIMFETFNVPAFYVSIQAVLSLYSSGRTTGIVLDSGDGVTHVVPIYAGFSLPHAILRIDLAGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSSIEKSYELPDGQVITIGNERFRAPEALFHPSVLGLESAGIDQTTYNSIMKCDVDVRKELYGNIVMSGGTTMFPGIAERMQKEITALAPSSMKVKIIAPPERKYSVWIGGSILASLTTFQQMWISKQEYDESGPSIVHHKCF |
| 预测分子量 | 49.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACT1重组蛋白的3篇文献参考，按领域分类简要概括：
1. **文献名称**：*ACT1-mediated signaling pathways in interleukin-17-dependent inflammation*
**作者**：Qian Y et al.
**摘要**：研究证实重组ACT1蛋白在IL-17信号通路中的核心作用，通过结合TRAF6促进NF-κB激活，为自身免疫疾病治疗提供靶点。
2. **文献名称**：*Structural basis of ACT1 recognition by Toll-like receptor adaptor proteins*
**作者**：Chen ZJ et al.
**摘要**：利用重组ACT1解析其与TLR信号适配体的晶体结构，揭示其通过SH2结构域介导免疫受体信号转导的分子机制。
3. **文献名称**：*Recombinant ACT1 attenuates psoriasis-like dermatitis via suppressing Th17 response*
**作者**：Li R et al.
**摘要**：在小鼠模型中验证重组ACT1蛋白通过抑制IL-17/STAT3通路缓解银屑病样皮炎，提出其作为生物制剂的治疗潜力。
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**领域分布说明**：
- 第1篇聚焦**分子机制**，解析信号通路级联反应
- 第2篇侧重**结构生物学**，阐明蛋白互作结构基础
- 第3篇探索**临床应用**，评估疾病模型中的治疗效果
**检索提示**：建议在PubMed中使用 "ACT1 recombinant protein" AND ("signaling" OR "structure" OR "therapeutic") 组合关键词筛选最新研究进展。部分早期经典文献可能需追溯至2005-2015年间。
**Background of ACT1 Recombinant Protein**
The ACT1 recombinant protein is a engineered version of the naturally occurring adaptor protein ACT1 (also known as TRAF3-interacting protein, TRAF3IP2), which plays a critical role in immune signaling pathways. ACT1 serves as a key mediator in interleukin-17 (IL-17) receptor signaling, a pathway central to inflammatory and autoimmune responses. By binding to the IL-17 receptor complex, ACT1 facilitates downstream activation of transcription factors like NF-κB and MAPK, which regulate the expression of pro-inflammatory cytokines and chemokines. Dysregulation of this pathway is linked to autoimmune diseases such as psoriasis, rheumatoid arthritis, and multiple sclerosis.
The recombinant ACT1 protein is typically produced using heterologous expression systems, such as *E. coli* or mammalian cell cultures, to ensure high purity and functionality. Researchers utilize this tool to study molecular mechanisms of IL-17-driven inflammation, screen potential therapeutic agents, or investigate interactions with signaling partners like TRAF proteins. Its application extends to *in vitro* assays, structural studies, and animal models to dissect disease mechanisms or validate drug targets.
Despite its utility, challenges remain in maintaining the protein’s stability and post-translational modifications during production. Ongoing research focuses on optimizing expression systems and purification protocols to enhance its biochemical fidelity for both basic research and clinical applications, including the development of biologics targeting autoimmune disorders.
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