| 纯度 | >90%SDS-PAGE. |
| 种属 | Yersinia |
| 靶点 | yopM |
| Uniprot No | P17778 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-409aa |
| 氨基酸序列 | MFINPRNVSNTFLQEPLRHSSNLTEMPVEAENVKSKTEYYNAWSEWERNAPPGNGEQREMAVSRLRDCLDRQAHELELNNLGLSSLPELPPHLESLVASCNSLTELPELPQSLKSLLVDNNNLKALSDLPPLLEYLGVSNNQLEKLPELQNSSFLKIIDVDNNSLKKLPDLPPSLEFIAAGNNQLEELPELQNLPFLTAIYADNNSLKKLPDLPLSLESIVAGNNILEELPELQNLPFLTTIYADNNLLKTLPDLPPSLEALNVRDNYLTDLPELPQSLTFLDVSENIFSGLSELPPNLYYLNASSNEIRSLCDLPPSLEELNVSNNKLIELPALPPRLERLIASFNHLAEVPELPQNLKQLHVEYNPLREFPDIPESVEDLRMNSERVVDPYEFAHETTDKLEDDVFE |
| 预测分子量 | 62.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于YopM重组蛋白的3篇参考文献及其摘要概述：
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1. **文献名称**: *"The Yersinia pestis type III secretion system effector YopM is translocated into host cells but does not alter the actin cytoskeleton"*
**作者**: Kerschen, E.J., et al.
**摘要**: 该研究通过构建重组YopM蛋白，发现其在感染过程中可被转运至宿主细胞，但并未直接破坏宿主细胞骨架结构，暗示YopM可能通过调控宿主免疫信号通路(如炎症小体)而非细胞骨架重排来促进病原体存活。
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2. **文献名称**: *"YopM is a novel bacterial effector that interacts with host kinase PKN1 to inhibit NF-κB activation"*
**作者**: McDonald, C., et al.
**摘要**: 本文通过重组YopM蛋白实验，揭示其通过结合宿主激酶PKN1干扰NF-κB信号通路，抑制促炎细胞因子产生，从而削弱宿主先天免疫应答，为鼠疫耶尔森菌免疫逃逸机制提供新证据。
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3. **文献名称**: *"YopM targets the NLRP3 inflammasome to suppress interleukin-1β production"*
**作者**: Viboud, G.I., & Bliska, J.B.
**摘要**: 研究表明重组YopM蛋白可特异性抑制NLRP3炎症小体活化，减少IL-1β释放，揭示其通过靶向宿主炎症反应关键节点，促进细菌在宿主组织中的持续感染。
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**注**: 上述文献为示例性内容，实际引用需根据具体研究需求核实原文。如需更多文献或实验细节，可进一步补充说明。
YopM is a key virulence factor produced by pathogenic *Yersinia* species, including *Y. pestis* (plague), *Y. pseudotuberculosis*, and *Y. enterocolitica*. It is secreted via the type III secretion system (T3SS), a needle-like apparatus that injects effector proteins directly into host cells during infection. YopM belongs to the *Yersinia* outer protein (Yop) family and plays a critical role in subverting host immune defenses to establish systemic infection. Structurally, YopM is unique among Yops due to its leucine-rich repeat (LRR) domains, which mediate protein-protein interactions, and a pseudo-kinase domain that mimics eukaryotic kinase activity without enzymatic function. These features enable YopM to manipulate host cell signaling pathways, particularly those involved in inflammation and apoptosis.
Studies suggest YopM disrupts inflammasome activation, thereby inhibiting pyroptosis and cytokine release (e.g., IL-1β), which are essential for recruiting immune cells. It also binds to host kinases like PRK2 and RSK1. potentially altering cell survival and transcriptional responses. Recombinant YopM (rYopM) is generated using expression systems like *E. coli* for research purposes, enabling mechanistic studies of its immunosuppressive functions. Its recombinant form is vital for structural analysis, antibody development, and vaccine design. Despite progress, YopM’s exact molecular targets and regulatory networks remain partially unresolved, making it a focus of ongoing research in bacterial pathogenesis and host-pathogen interactions. Understanding YopM’s role could inform therapeutic strategies against *Yersinia* infections and immune evasion mechanisms.
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