| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | PrgJ |
| Uniprot No | P41785 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-101aa |
| 氨基酸序列 | MSIATIVPENAVIGQAVNIRSMETDIVSLDDRLLQAFSGSAIATAVDKQTITNRIEDPNLVTDPKELAISQEMISDYNLYVSMVSTLTRKGVGAVETLLRS |
| 预测分子量 | 12.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PrgJ重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: *"Structural characterization of the PrgJ protein from the Salmonella enterica SPI-1 type III secretion system"*
**作者**: Kubori, T., et al.
**摘要**: 该研究解析了沙门氏菌III型分泌系统(T3SS)中PrgJ蛋白的晶体结构，揭示了其作为针状复合体组件的功能，并探讨其与宿主细胞膜互作的机制，为理解病原体侵袭机制提供结构基础。
2. **文献名称**: *"PrgJ: A key component in plasmid conjugation and virulence regulation in Enterococcus faecalis"*
**作者**: Chen, J., et al.
**摘要**: 文章报道了肠球菌质粒pCF10中PrgJ蛋白的双重功能：作为接合转移分泌组分促进质粒传递，同时作为信号肽调控毒力基因表达，通过重组蛋白实验验证了其与宿主受体的相互作用。
3. **文献名称**: *"Functional analysis of recombinant PrgJ in bacterial type III secretion system assembly"*
**作者**: Tampakaki, A.P., et al.
**摘要**: 研究通过体外重组表达PrgJ，证明其与PrgI、PrgK等蛋白共同形成T3SS跨膜通道，并利用基因敲除实验表明PrgJ缺失会导致分泌系统功能缺陷，影响沙门氏菌的宿主细胞侵袭能力。
---
**备注**：以上文献信息为示例性概括，实际文献需通过数据库(如PubMed、Web of Science)以“PrgJ”、“type III secretion system”或“bacterial conjugation”为关键词检索获取。PrgJ的研究多聚焦于其在分泌系统结构或质粒转移中的机制。
PrgJ recombinant protein is derived from the PrgJ antigen, a component of the type III secretion system (T3SS) in *Salmonella enterica* and related Gram-negative bacteria. The T3SS is a needle-like apparatus critical for bacterial pathogenesis, enabling the injection of virulence factors into host cells to manipulate cellular processes and establish infection. PrgJ, encoded by the *prgJ* gene within the *Salmonella* pathogenicity island 1 (SPI-1), is a structural protein contributing to the assembly of the T3SS needle complex. It plays a role in stabilizing the secretion machinery and facilitating effector protein translocation.
Recombinant PrgJ is produced through genetic engineering, typically by cloning the *prgJ* gene into expression vectors (e.g., *E. coli* systems) followed by purification. Its study provides insights into T3SS architecture, bacterial-host interactions, and virulence mechanisms. Researchers utilize PrgJ recombinant protein to investigate immune responses, as it can serve as an antigen to elicit antibody production or T-cell activation in model systems. This has implications for vaccine development, particularly in designing subunit vaccines targeting bacterial secretion systems.
Additionally, PrgJ is a tool for structural biology; its crystallization and biophysical characterization help elucidate molecular interactions within the T3SS. Beyond basic research, inhibitors targeting PrgJ or its interactions are explored for antimicrobial therapies, aiming to disrupt pathogenicity without promoting antibiotic resistance. Overall, PrgJ recombinant protein bridges fundamental microbiology and translational applications, offering a platform to dissect infection strategies and develop novel anti-infective strategies.
×
