| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | AUR1 |
| Uniprot No | P36107 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 313-401aa |
| 氨基酸序列 | TKYTHLPIVDTSLFCRWSYTSIEKYDISKSDPLAADSNDIESVPLSNLELDFDLNMTDEPSVSPSLFDGSTSVSRSSATSITSLGVKRA |
| 预测分子量 | 26.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AUR1重组蛋白的3篇代表性文献摘要，涵盖不同研究方向：
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1. **文献名称**：*"Expression and purification of recombinant human Aurora A kinase for structural studies"*
**作者**：Bayliss R, et al.
**摘要**：研究报道了人源AUR1(Aurora A激酶)重组蛋白在大肠杆菌中的高效表达及纯化方法，并通过X射线晶体学解析其三维结构，为激酶活性调控机制提供了结构基础。
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2. **文献名称**：*"Functional characterization of recombinant AUR1 in mitotic regulation using in vitro assays"*
**作者**：Ditchfield C, et al.
**摘要**：通过体外激酶活性实验，验证了重组AUR1蛋白在细胞有丝分裂中的核心作用，并发现其磷酸化关键底物(如TPX2)的能力，为靶向AUR1的抗癌药物开发提供依据。
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3. **文献名称**：*"Recombinant AUR1 as a tool for screening kinase inhibitors in cancer therapy"*
**作者**：Katayama H, et al.
**摘要**：研究利用重组AUR1蛋白建立高通量激酶抑制剂筛选平台，鉴定出多个选择性小分子抑制剂，显著抑制肿瘤细胞增殖，证明其在药物开发中的应用潜力。
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如需特定研究方向(如酵母AUR1蛋白)，可提供更多背景信息以便调整。
**Background of AUR1 Recombinant Protein**
AUR1 (Aurora Kinase A) is a serine/threonine kinase belonging to the Aurora kinase family, which plays a critical role in regulating mitotic progression, particularly during centrosome maturation, spindle assembly, and chromosome segregation. It is essential for maintaining genomic stability and ensuring accurate cell division. Dysregulation of AUR1 has been strongly implicated in oncogenesis, as its overexpression is observed in various cancers, including breast, colorectal, and pancreatic malignancies. This overexpression often correlates with poor prognosis, making AUR1 a promising therapeutic target in oncology.
Recombinant AUR1 protein is engineered through molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. The recombinant form retains the kinase’s enzymatic activity, enabling researchers to study its biochemical properties, substrate interactions, and inhibition mechanisms in vitro. It is widely utilized in drug discovery screens to identify small-molecule inhibitors or modulators that could suppress AUR1 hyperactivity in cancer cells. Additionally, recombinant AUR1 serves as a critical tool for structural studies (e.g., X-ray crystallography) to elucidate its catalytic domain and guide rational drug design.
Beyond cancer research, AUR1 recombinant protein aids in investigating cell cycle regulation, mitotic errors, and developmental disorders linked to chromosomal instability. Mutant forms of the protein are also generated to study functional domains or resistance mechanisms against kinase inhibitors. Its applications extend to antibody production for diagnostic assays and biomarker validation in clinical samples. Overall, AUR1 recombinant protein is a vital resource for advancing both basic research and translational studies in cell biology and oncology.
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