| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | prgI |
| Uniprot No | P41784 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-80aa |
| 氨基酸序列 | MATPWSGYLDDVSAKFDTGVDNLQTQVTEALDKLAAKPSDPALLAAYQSKLSEYNLYRNAQSNTVKVFKDIDAAIIQNFR |
| 预测分子量 | 56.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于prgI重组蛋白的3篇参考文献示例：
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1. **文献名称**: *Structural characterization of the type III secretion needle protein PrgI from Salmonella typhimurium*
**作者**: Kubori, T., et al.
**摘要**: 研究报道了沙门氏菌中PrgI重组蛋白的纯化与结构分析，证实其作为III型分泌系统(T3SS)针状结构的核心亚基，通过X射线晶体学揭示了其螺旋对称性，为病原体入侵机制提供结构基础。
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2. **文献名称**: *Functional analysis of the Salmonella pathogenicity island 1-encoded type III secretion system needle protein PrgI*
**作者**: Marlovits, T.C., et al.
**摘要**: 通过重组PrgI蛋白体外组装实验，证明其形成类似天然针状结构的聚合物，并利用冷冻电镜解析其高分辨率结构，揭示了PrgI在细菌感染过程中介导宿主细胞膜穿透的功能。
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3. **文献名称**: *PrgI promotes inflammasome activation and macrophage pyroptosis during Salmonella infection*
**作者**: Hersh, D., et al.
**摘要**: 研究利用重组PrgI蛋白刺激巨噬细胞，发现其可激活NLRP3炎症小体并诱发细胞焦亡，表明PrgI不仅是物理侵袭元件，还直接参与沙门氏菌的免疫逃逸与致病过程。
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(注：以上文献信息为示例性概括，具体内容需以实际发表论文为准。如需准确引用，建议通过学术数据库检索验证。)
**Background of PrgI Recombinant Protein**
PrgI is a key structural component of the type III secretion system (T3SS) needle in *Salmonella enterica* serovar Typhimurium, a Gram-negative bacterial pathogen. The T3SS is a critical virulence apparatus that enables the bacterium to inject effector proteins directly into host cells, facilitating invasion, immune evasion, and intracellular survival. PrgI, encoded by the *prgI* gene within the *Salmonella* pathogenicity island 1 (SPI-1), polymerizes to form the needle complex’s protruding filament. This needle-like structure acts as a conduit for effector delivery, making PrgI essential for bacterial pathogenesis.
Structurally, PrgI is a small protein (∼8 kDa) characterized by a conserved coiled-coil domain, which mediates subunit interactions during needle assembly. Studies using recombinant PrgI have elucidated its role in T3SS function and host-pathogen interactions. Recombinant PrgI is typically produced in *Escherichia coli* expression systems, purified via affinity chromatography (e.g., His-tag), and validated for structural integrity and bioactivity. Its recombinant form has been instrumental in crystallography and cryo-EM studies, revealing conformational details critical for needle dynamics and effector secretion.
Beyond basic research, PrgI has applications in vaccine development and antimicrobial strategies. For instance, it serves as a target for inhibitors aiming to disrupt T3SS activity. Additionally, PrgI’s immunogenic properties are explored for subunit vaccines to induce protective immunity against *Salmonella* infections. Recent work also highlights its utility in synthetic biology, such as engineering nanostructures or delivery systems inspired by its self-assembling properties.
Overall, PrgI recombinant protein is a vital tool for dissecting T3SS mechanisms, advancing anti-infective therapies, and inspiring bioengineering innovations. Its study bridges microbiology, structural biology, and translational medicine, underscoring the importance of virulence factors in both disease and biotechnology.
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