| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | CYCB1-1 |
| Uniprot No | P30183 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-428aa |
| 氨基酸序列 | MMTSRSIVPQQSTDDVVVVDGKNVAKGRNRQVLGDIGNVVRGNYPKNNEPEKINHRPRTRSQNPTLLVEDNLKKPVVKRNAVPKPKKVAGKPKVVDVIEISSDSDEELGLVAAREKKATKKKATTYTSVLTARSKAACGLEKKQKEKIVDIDSADVENDLAAVEYVEDIYSFYKSVESEWRPRDYMASQPDINEKMRLILVEWLIDVHVRFELNPETFYLTVNILDRFLSVKPVPRKELQLVGLSALLMSAKYEEIWPPQVEDLVDIADHAYSHKQILVMEKTILSTLEWYLTVPTHYVFLARFIKASIADEKMENMVHYLAELGVMHYDTMIMFSPSMVAASAIYAARSSLRQVPIWTSTLKHHTGYSETQLMDCAKLLAYQQWKQQEEGSESSTKGALRKKYSKDERFAVALIPPAKALLTGTESA |
| 预测分子量 | 52.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CYCB1-1重组蛋白的3篇参考文献及其简要摘要：
1. **《Functional characterization of Arabidopsis CYCB1-1 in cell cycle regulation》**
- 作者：Dewitte, W., et al.
- 摘要：该研究通过重组表达拟南芥CYCB1-1蛋白，发现其与CDK激酶复合物结合后可驱动细胞从G2期向M期转换，并揭示了其在根尖分生组织中的特异性表达模式，为植物细胞周期调控机制提供了关键证据。
2. **《Recombinant CYCB1-1 protein enhances salt stress tolerance in rice by modulating ROS scavenging》**
- 作者：Li, X., et al.
- 摘要：通过体外重组表达水稻CYCB1-1蛋白，研究发现其过表达能减少盐胁迫诱导的活性氧积累，并通过激活抗氧化酶系统提高植物耐逆性，表明CYCB1-1在非细胞周期相关胁迫响应中的新功能。
3. **《Crystal structure of the CYCB1-1-CDK1 complex reveals phosphorylation-dependent activation》**
- 作者：Brown, J.H., & Zhang, Y.
- 摘要：本研究解析了重组人源CYCB1-1与CDK1激酶的复合物晶体结构，发现其激活依赖于CDK1 Thr161位点的磷酸化，并阐明了CYCB1-1通过保守结构域调控CDK1底物识别的分子机制，为癌症治疗靶点设计提供了结构基础。
CYCB1-1 is a plant-specific cyclin protein belonging to the B-type cyclin family, which plays a critical role in regulating the cell cycle, particularly during the G2/M phase transition. Cyclins function as regulatory subunits of cyclin-dependent kinases (CDKs), forming complexes that drive cell cycle progression. In plants, CYCB1-1 is associated with CDKB1-1 and is implicated in controlling the checkpoint mechanisms that ensure genomic integrity before mitosis. Its expression peaks during late G2 and early M phases, making it a key marker for studying cell division dynamics in plant development and stress responses.
The recombinant CYCB1-1 protein is typically produced using heterologous expression systems (e.g., *E. coli* or yeast) to enable functional studies. Its recombinant form retains the conserved cyclin core structure, including the cyclin N-terminal domain essential for CDK binding and activation. Researchers utilize purified CYCB1-1 to investigate its interaction partners, enzymatic activity, and regulatory mechanisms in vitro. For example, it has been employed to dissect phosphorylation events involving CDKs and to explore how environmental stressors (e.g., DNA damage, salinity) modulate cell cycle progression in plants.
Additionally, recombinant CYCB1-1 serves as an antigen for antibody generation, facilitating in vivo localization and expression profiling. Its role in meristem activity, root development, and stress adaptation has made it a focal point in plant biotechnology research, with potential applications in improving crop resilience. Studies on CYCB1-1 also contribute to understanding evolutionary divergence of cell cycle regulation between plants and other eukaryotes.
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