| 纯度 | >90%SDS-PAGE. |
| 种属 | Arabidopsis |
| 靶点 | XERO1 |
| Uniprot No | P25863 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-128aa |
| 氨基酸序列 | MESYQNQSGAQQTHQQLDQFGNPFPATTGAYGTAGGAPAVAEGGGLSGMLHRSGSSSSSSSEDDGLGGRRRKKKGITEKIKEKLPGHHDSNKTSSLGSTTTAYDTGTVHHEKKGMMEKIKEKLPGGHH |
| 预测分子量 | 15.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为假设性参考文献示例(XERO1为虚构名称，实际文献需根据具体研究领域检索)：
1. **"Cloning and Expression of XERO1 Recombinant Protein in E. coli"**
- 作者：Smith A, et al.
- 摘要：研究成功克隆XERO1基因并在大肠杆菌中实现高效表达，通过亲和层析纯化获得功能性重组蛋白，验证其体外酶活性。
2. **"Structural Analysis of XERO1 in Plant Stress Response"**
- 作者：Wang L, et al.
- 摘要：解析XERO1重组蛋白的晶体结构，揭示其通过结合特定信号分子参与植物干旱胁迫应答的分子机制。
3. **"XERO1 Recombinant Protein Enhances Drug Delivery Efficiency"**
- 作者：Chen R, et al.
- 摘要：利用重组XERO1蛋白修饰纳米颗粒，显著提高靶向药物在肿瘤细胞中的穿透性和递送效率。
4. **"Functional Characterization of XERO1 in Immune Regulation"**
- 作者：Garcia M, et al.
- 摘要：通过体外实验证实重组XERO1蛋白能够调控巨噬细胞极化，为免疫相关疾病治疗提供潜在靶点。
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**注意**：以上文献为模拟内容，实际研究中需通过PubMed、Web of Science等平台检索具体关键词(如“XERO1 recombinant protein”或相关功能描述)。若XERO1为特定领域术语，建议提供更多背景信息以便精准查找。
**Background of XERO1 Recombinant Protein**
XERO1 recombinant protein is a genetically engineered molecule designed to replicate the functional properties of the native XERO1 protein, which plays a critical role in cellular processes such as stress response, signaling, or enzymatic regulation (specific functions depend on the organism and context). The term "recombinant" indicates that the protein is produced using recombinant DNA technology, where the XERO1 gene is cloned into an expression vector and introduced into host cells (e.g., *E. coli*, yeast, or mammalian cells) for large-scale production. This approach allows for high-purity yields, consistency, and customization (e.g., tagging for detection or purification).
Native XERO1 is often associated with drought tolerance in plants or DNA repair mechanisms in certain organisms, though its exact biological role varies. For instance, in plant studies, XERO1 homologs may regulate abscisic acid (ABA) pathways or stomatal closure under water-deficient conditions. Recombinant XERO1 enables researchers to study its structure-function relationships, interaction partners, or biochemical activity *in vitro*. It also holds potential applications in biotechnology, such as developing stress-resistant crops or therapeutic agents targeting pathways where XERO1 is implicated (e.g., cancer or neurodegenerative diseases).
Characterization typically involves techniques like SDS-PAGE, Western blotting, and mass spectrometry to confirm identity and purity. Functional assays (e.g., enzyme activity tests or cell-based models) validate its bioactivity. Ongoing research focuses on optimizing expression systems and exploring XERO1’s therapeutic or agricultural relevance, highlighting its importance in both basic science and applied biotechnology.
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