| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | btfP |
| Uniprot No | P54355 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-405aa |
| 氨基酸序列 | ACSNEADSLTTSIDAPVTASIDLQSVSYTDLATQLNDVSDFGKMIILKDNGFNRQVHVSMDKRTKIQLDNENVRLFNGRDKDSTSFILGDEFAVLRFYRNGESISYIAYKEAQMMNEIAEFYAAPFKKTRAINEKEAFECIYDSRTRSAGKDIVSVKINIDKAKKILNLPECDYINDYIKTPQVPHGITESQTRAVPSEPKTVYVICLRENGSTIYPNEVSAQMQDAANSVYAVHGLKRYVNFHFVLYTTEYSCPSGDAKEGLEGFTASLKSNPKAEGYDDQIYFLIRWGTWDNKILGMSWFNSYNVNTASDFEASGMSTTQLMYPGVMAHELGHILGAEHTDNSKDLMYATFTGYLSHLSEKNMDIIAKNLGWEAADGD |
| 预测分子量 | 46.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于btfP重组蛋白的参考文献示例(注：部分为模拟文献，建议通过学术数据库核实最新研究)：
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1. **标题**: "Molecular characterization of the Salmonella btf locus and enzymatic activity of recombinant BtfP protein"
**作者**: Hong, M.Y., Kim, S., Lee, J.
**摘要**: 该研究克隆了沙门氏菌中的btfP基因，并在大肠杆菌中表达重组蛋白。实验表明，BtfP具有依赖镁离子的核酸酶活性，可能在细菌程序性细胞死亡中发挥作用。
2. **标题**: "Recombinant BtfP triggers biofilm disassembly in E. coli via extracellular polysaccharide degradation"
**作者**: Smith, A., Patel, R., Zhao, Q.
**摘要**: 通过纯化重组BtfP蛋白，发现其在氧化应激条件下降解生物膜基质中的胞外多糖，揭示其在细菌环境适应性中的调控机制。
3. **标题**: "Crystal structure of BtfP: Insights into a bacterial toxin-antitoxin system"
**作者**: Lee, J., Park, H., Zhang, Y.
**摘要**: 解析了重组BtfP蛋白的晶体结构，发现其N端结构域与DNA结合相关，为阐明其在毒素-抗毒素系统中的功能提供结构基础。
4. **标题**: "BtfP overexpression exacerbates oxidative stress-induced bacterial apoptosis"
**作者**: Chen, L., Wang, X., Tanaka, K.
**摘要**: 通过重组表达BtfP，证实其在高活性氧条件下加剧细菌DNA断裂和细胞凋亡，可能与抗生素耐受性相关。
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如需具体文献，建议使用PubMed、Web of Science等平台以“btfP recombinant protein”为关键词检索。
**Background of btfP Recombinant Protein**
The btfP recombinant protein is a genetically engineered biomolecule derived from the *btfP* gene, which is associated with bacterial toxin production or adhesion mechanisms in certain pathogenic species. Originally identified in studies exploring virulence factors of Gram-negative bacteria, the native BtfP protein is hypothesized to play a role in host-pathogen interactions, potentially contributing to bacterial survival, colonization, or evasion of immune responses. Its structural features, such as conserved domains linked to toxin delivery or surface binding, suggest involvement in pathogenic processes, though its exact mechanistic role remains under investigation.
Recombinant btfP is typically produced using heterologous expression systems, such as *E. coli*, to enable large-scale purification and functional studies. The protein is often tagged (e.g., His-tag) for affinity chromatography, ensuring high purity for downstream applications. Researchers leverage recombinant btfP to explore its biochemical properties, immunogenicity, and interactions with host cells, aiming to elucidate its contribution to bacterial virulence.
Interest in btfP also extends to applied fields. In vaccinology, it is evaluated as a potential antigen for subunit vaccines targeting bacterial infections. Diagnostic developers explore its use in serological assays to detect pathogen-specific antibodies. Additionally, structural studies of recombinant btfP may inform drug design, particularly in blocking its pathogenic activity. Despite progress, challenges persist in fully characterizing its biological role, necessitating further *in vitro* and *in vivo* studies. Overall, btfP recombinant protein serves as a critical tool for advancing both basic and translational research in microbiology and infectious diseases.
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