| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | rdgB |
| Uniprot No | P52061 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-197aa |
| 氨基酸序列 | MQKVVLATGNVGKVRELASLLSDFGLDIVAQTDLGVDSAEETGLTFIENAILKARHAAKVTALPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKLLETMKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIFFVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG |
| 预测分子量 | 41.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RDGB(Retinal Degeneration B)重组蛋白的假设性参考文献示例(基于领域知识构建，非真实文献)：
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1. **文献名称**: *Functional characterization of recombinant Drosophila RDGB protein in phospholipid signaling*
**作者**: Smith J, et al.
**摘要**: 本研究成功克隆并表达了果蝇RDGB重组蛋白，通过体外脂质结合实验证明其具有磷脂酰肌醇转移活性，并揭示了其在光感受器细胞膜脂质稳态中的关键作用。
2. **文献名称**: *Crystal structure of the RDGB-PH domain and its interaction with phosphoinositides*
**作者**: Lee H, et al.
**摘要**: 解析了RDGB蛋白的PH结构域晶体结构，通过表面等离子共振技术验证了其与PIP2的特异性结合，为解释rdgB突变导致视网膜退化的分子机制提供结构基础。
3. **文献名称**: *Role of RDGB-α in maintaining TRP channel activity during phototransduction*
**作者**: Wang Y, et al.
**摘要**: 利用重组RDGB-α蛋白与TRP通道共表达系统，证明其通过调控DAG代谢维持通道活性，揭示了其在果蝇视觉信号转导中的脂质第二信使缓冲功能。
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注：以上文献为领域知识模拟，实际引用时请通过PubMed/Web of Science检索真实文献(关键词：RDGB, retinal degeneration, phosphatidylinositol transfer protein)。
The rdgB (Retinal Degeneration B) protein, first identified in Drosophila melanogaster, plays a critical role in phototransduction and retinal maintenance. Mutations in the rdgB gene lead to rapid light-dependent retinal degeneration in flies, highlighting its importance in visual signaling and cellular homeostasis. Structurally, rdgB is a membrane-associated protein containing an N-terminal phosphatidylinositol transfer domain (PITP), a central transmembrane region, and C-terminal lipid-binding domains. It facilitates lipid metabolism by transferring phosphatidylinositol (PI) between membranes, supporting the regeneration of phosphatidylinositol 4.5-bisphosphate (PIP2), a key signaling molecule in photoreceptor cells.
In mammals, the rdgB homologs, PITPNA and PITPNB, share functional similarities, influencing phospholipid trafficking and membrane dynamics. Dysregulation of these proteins is linked to retinal pathologies, including inherited degenerative diseases. Recombinant rdgB proteins, produced via heterologous expression systems (e.g., E. coli or mammalian cells), enable biochemical and structural studies to dissect their lipid-binding/transfer mechanisms and interactions with signaling pathways.
Research using recombinant rdgB has clarified its role in maintaining photoreceptor integrity by replenishing PIP2 pools depleted during light activation. Additionally, it aids in modeling disease mechanisms and screening therapeutic compounds targeting lipid metabolism defects. Recent studies explore its potential in gene therapy to rescue retinal degeneration phenotypes. Despite progress, questions remain about its regulation and tissue-specific functions, underscoring the need for further investigation. Overall, rdgB recombinant proteins serve as vital tools for understanding retinal biology and developing treatments for vision disorders.
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