| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | At3g57050 |
| Uniprot No | P53780 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 70-464aa |
| 氨基酸序列 | NNTTDSLNTMNIKEEASVSTLLVNLDNKFDPFDAMSTPLYQTATFKQPSAIENGPYDYTRSGNPTRDALESLLAKLDKADRAFCFTSGMAALSAVTHLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLDEVAAAIGPQTKLVWLESPTNPRQQISDIRKISEMAHAQGALVLVDNSIMSPVLSRPLELGADIVMHSATKFIAGHSDVMAGVLAVKGEKLAKEVYFLQNSEGSGLAPFDCWLCLRGIKTMALRIEKQQENARKIAMYLSSHPRVKKVYYAGLPDHPGHHLHFSQAKGAGSVFSFITGSVALSKHLVETTKYFSIAVSFGSVKSLISMPCFMSHASIPAEVREARGLTEDLVRISAGIEDVDDLISDLDIAFKTFPL |
| 预测分子量 | 46.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于拟南芥At3g57050重组蛋白的假设性参考文献示例，基于该基因可能的生物学功能(如编码dUTPase)和相关研究方向的合理推测：
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1. **"Heterologous Expression and Biochemical Characterization of Arabidopsis dUTPase (At3g57050)"**
*作者：Miller R, et al.*
**摘要**：研究在大肠杆菌中成功表达并纯化了At3g57050重组蛋白，证实其具有dUTP水解酶活性，并分析了其酶动力学参数(如Km和Vmax)，为理解其在植物DNA代谢中的作用提供依据。
2. **"Crystal Structure of At3g57050 Reveals Conserved dUTPase Motifs"**
*作者：Chen L, et al.*
**摘要**：通过X射线晶体学解析了At3g57050重组蛋白的3D结构，发现其具有典型的dUTPase折叠模式，并鉴定了关键催化残基，为靶向酶活性调控提供结构基础。
3. **"At3g57050 Knockout Mutants Exhibit Developmental Defects via Altered dUTP Metabolism"**
*作者：Wang X, et al.*
**摘要**：结合重组蛋白体外实验和突变体分析，证明At3g57050通过调控dUTP水平维持基因组稳定性，缺失该基因导致拟南芥根尖分生组织异常。
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**注意**：以上文献为示例性质，实际研究中请通过PubMed、Google Scholar或TAIR数据库检索关键词(如“At3g57050 recombinant protein”“Arabidopsis dUTPase”)获取真实文献。
At3g57050. a gene in *Arabidopsis thaliana*, encodes a cytochrome P450 monooxygenase designated as CYP71B34. This enzyme belongs to the CYP71 clan, one of the largest plant P450 families involved in specialized metabolism. Cytochrome P450s are heme-containing proteins that catalyze oxidation reactions, often critical for synthesizing secondary metabolites, phytohormones, or detoxifying compounds. CYP71B34 is associated with plant defense mechanisms, particularly in response to biotic stress. Studies suggest its involvement in the biosynthesis or modification of defense-related metabolites, such as camalexin or glucosinolates, though its exact substrates remain under investigation.
The recombinant protein of At3g57050 is typically expressed in heterologous systems like *E. coli* or yeast for functional characterization. Structural predictions indicate conserved P450 features: a hydrophobic N-terminal membrane anchor, a α-helix-rich globular domain, and a heme-binding region. Its activity is NADPH- and oxygen-dependent, common to P450s. Research highlights its upregulated expression during pathogen attacks, notably by *Pseudomonas syringae* or fungal pathogens, implying a role in antimicrobial compound production.
Functional studies using recombinant CYP71B34 have explored its catalytic versatility. In vitro assays reveal potential involvement in hydroxylation or oxidative tailoring of aromatic compounds. Interaction with redox partners like cytochrome b5 is hypothesized to enhance catalytic efficiency. Despite progress, its physiological context—whether it acts in tandem with specific pathways (e.g., tryptophan-derived metabolites) or regulates cross-talk between defense signals—requires deeper exploration.
Overall, At3g57050 recombinant protein serves as a tool to dissect plant metabolic adaptations to stress, offering insights into engineering disease-resistant crops or biotechnological synthesis of bioactive molecules.
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