| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | Rv1984c |
| Uniprot No | P9WP43 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 33-217aa |
| 氨基酸序列 | DPCSDIAVVFARGTHQASGLGDVGEAFVDSLTSQVGGRSIGVYAVNYPASDDYRASASNGSDDASAHIQRTVASCPNTRIVLGGYSQGATVIDLSTSAMPPAVADHVAAVALFGEPSSGFSSMLWGGGSLPTIGPLYSSKTINLCAPDDPICTGGGNIMAHVSYVQSGMTSQAATFAANRLDHAG |
| 预测分子量 | 38.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Rv1984c重组蛋白的3条示例参考文献(注：文献信息为模拟示例，实际引用需核实原文)：
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1. **文献名称**: *Immunogenicity of Mycobacterium tuberculosis recombinant protein Rv1984c in a murine model*
**作者**: Zhang Y, et al.
**摘要**: 研究通过在大肠杆菌中表达Rv1984c重组蛋白，发现其在小鼠模型中能诱导强烈的Th1型免疫反应(IFN-γ和IL-2升高)，并显著降低结核分枝杆菌的肺部载菌量，提示其作为疫苗候选分子的潜力。
2. **文献名称**: *Rv1984c as a novel serodiagnostic antigen for active tuberculosis: A multi-cohort evaluation*
**作者**: Singh A, et al.
**摘要**: 评估Rv1984c重组蛋白在活动性结核病血清诊断中的应用。研究显示，该抗原对结核患者的血清IgG抗体检测敏感性和特异性分别达78%和93%，尤其在涂片阴性病例中表现优于传统抗原ESAT-6/CFP-10.
3. **文献名称**: *Structural and functional characterization of Rv1984c-encoded peptidase from Mycobacterium tuberculosis*
**作者**: Kumar D, et al.
**摘要**: 解析了Rv1984c蛋白的晶体结构，证实其属于金属依赖型肽酶家族，并通过体外实验验证其水解特定肽底物的活性。研究推测该蛋白可能通过调控宿主-病原体相互作用参与结核分枝杆菌的致病机制。
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建议通过PubMed、Google Scholar等平台以关键词“Rv1984c recombinant protein”或“Rv1984c antigen”检索最新文献以获取准确信息。
**Background of the Rv1984c Recombinant Protein**
The Rv1984c gene, encoded by *Mycobacterium tuberculosis* (Mtb), is associated with the pathogen’s virulence and survival mechanisms. This gene is part of the *mce3* operon, which plays a role in host cell invasion and immune modulation. The Rv1984c protein is a secreted antigen, hypothesized to interact with host immune systems during infection, potentially influencing macrophage responses and cytokine signaling. Its expression is upregulated under stress conditions, suggesting involvement in bacterial adaptation during host-pathogen interactions.
Recombinant Rv1984c protein is produced via genetic engineering, typically by cloning the gene into expression vectors (e.g., *E. coli*) followed by purification. This approach allows large-scale production for functional and immunological studies. Research highlights its immunogenicity, eliciting robust antibody and T-cell responses in animal models, indicating potential as a diagnostic or vaccine candidate. Studies show Rv1984c-specific antibodies in TB patients’ sera, supporting its relevance in humoral immunity.
In diagnostics, Rv1984c has been evaluated in antigen-based assays, demonstrating moderate sensitivity and specificity compared to traditional TB markers. For vaccine development, its inclusion in multi-antigen formulations aims to enhance protective immunity. However, challenges remain, such as cross-reactivity with non-tuberculous mycobacteria and variable immune recognition across populations.
Ongoing research focuses on elucidating Rv1984c’s precise biological role, optimizing its use in diagnostic kits, and exploring synergy with other antigens (e.g., ESAT-6/CFP-10) to improve TB detection or vaccine efficacy. Its study contributes to understanding Mtb pathogenesis and advancing targeted therapeutic strategies.
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