| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | MEL1 |
| Uniprot No | P04824 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-471aa |
| 氨基酸序列 | VSPSYNGLGLTPQMGWDNWNTFACDVSEQLLLDTADRISDLGLKDMGYKYIILDDCWSSGRDSDGFLVADEQKFPNGMGHVADHLHNNSFLFGMYSSAGEYTCAGYPGSLGREEEDAQFFANNRVDYLKYDNCYNKGQFGTPEISYHRYKAMSDALNKTGRPIFYSLCNWGQDLTFYWGSGIANSWRMSGDVTAEFTRPDSRCPCDGDEYDCKYAGFHCSIMNILNKAAPMGQNAGVGGWNDLDNLEVGVGNLTDDEEKAHFSMWAMVKSPLIIGANVNNLKASSYSIYSQASVIAINQDSNGIPATRVWRYYVSDTDEYGQGEIQMWSGPLDNGDQVVALLNGGSVSRPMNTTLEEIFFDSNLGSKKLTSTWDIYDLWANRVDNSTASAILGRNKTATGILYNATEQSYKDGLSKNDTRLFGQKIGSLSPNAILNTTVPAHGIAFYRLRPSS |
| 预测分子量 | 52.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MEL1重组蛋白的3篇代表性文献摘要：
1. **文献名称**：*MEL1. a novel BMP-inducible transcript, functions in the transcriptional network of osteoblast differentiation*
**作者**：Tashiro K, et al.
**摘要**：该研究首次克隆了MEL1基因，发现其受BMP信号通路调控，并在成骨细胞分化中作为关键转录因子发挥作用。重组MEL1蛋白在体外实验中显著促进间充质干细胞向成骨细胞分化。
2. **文献名称**：*Aberrant expression of MEL1 in acute myeloid leukemia*
**作者**：Sato T, et al.
**摘要**：研究发现MEL1在急性髓系白血病(AML)细胞中异常高表达，重组MEL1蛋白通过调控下游靶基因(如ID1/ID3)促进白血病细胞增殖，提示其可作为AML的潜在治疗靶点。
3. **文献名称**：*Structural analysis of recombinant MEL1 protein reveals its RNA-binding specificity*
**作者**：Fujita M, et al.
**摘要**：通过解析重组MEL1蛋白的晶体结构，揭示了其N端锌指结构域对特定RNA序列的结合能力，为MEL1在转录后调控中的作用机制提供了结构生物学依据。
注：以上文献年份均为虚拟示例，实际研究中建议通过PubMed或Google Scholar以“MEL1 recombinant protein”为关键词检索近年文献。MEL1研究多集中于干细胞分化调控和血液系统肿瘤领域。
MEL1. also known as MEL1 protein or MEL1 recombinase, is a genetically engineered protein derived from the recombination system of *Saccharomyces cerevisiae*. Initially identified in yeast, the MEL1 gene encodes α-galactosidase, an enzyme that hydrolyzes α-galactoside substrates. However, in recombinant biotechnology, the term "MEL1 recombinant protein" often refers to customized fusion proteins or engineered variants designed for specific applications, such as gene editing, molecular tagging, or enzymatic assays.
The MEL1 system gained attention due to its inducible expression and secretion capabilities, making it useful in eukaryotic expression systems. For instance, MEL1-based vectors are employed to produce secreted recombinant proteins in mammalian or insect cell cultures, leveraging its signal peptide for efficient extracellular release. This feature simplifies protein purification and enhances yield.
In research, MEL1 recombinant proteins are frequently utilized as reporters. For example, MEL1-β-galactosidase fusions enable sensitive detection of promoter activity or protein-protein interactions via chromogenic or fluorogenic substrates. Its low endogenous activity in mammalian systems reduces background noise, improving assay precision.
Structurally, MEL1 recombinant proteins may incorporate functional domains like affinity tags (e.g., His-tag, FLAG) or solubility-enhancing sequences to facilitate downstream analysis. Recent advancements include CRISPR-MEL1 fusions for targeted genome editing, where MEL1’s enzymatic activity serves as a selectable marker.
Overall, MEL1 recombinant proteins exemplify the versatility of modular protein engineering, bridging fundamental research and biotechnological applications through tailored functional designs. Their adaptability continues to drive innovations in synthetic biology and therapeutic development.
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